Comparison of single versus booster dose of influenza vaccination on humoral and cellular immune responses in older adults

被引:39
作者
McElhaney, JE
Hooton, JW
Hooton, N
Bleackley, RC
机构
[1] Univ Connecticut, Sch Med, Ctr Immunotherapy Canc & Infect Dis, Farmington, CT 06030 USA
[2] Univ Alberta, Dept Med, Edmonton, AB, Canada
[3] Univ Alberta, Dept Biochem, Edmonton, AB, Canada
关键词
cytokines; granzyme B; influenza vaccination; older adults;
D O I
10.1016/j.vaccine.2005.01.080
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
This study compared the immune response to the standard single-dose (SD) of influenza vaccine to a booster dose (BD) re-vaccination given 16 weeks after the initial dose. While seroprotection rates following vaccination were similar, T-cell responses were more optimally stimulated in the SD versus the BD group. SD lead to a greater than 10-fold decline in ex vivo interleukin-10 (IL-10) levels (P < .0001) and a corresponding significant increase in the interferon-gamma (IFN-gamma) to IL-10 ratio. Although BD had no further effect on IL-10 production, the IFN-gamma:IL-10 ratio declined in the BD group (P < .001, A/H3N2 and B strains). In the SD group only, IFN-gamma:IL-10 ratios significantly correlated with serum antibody titers (R = .37-.50, P < .01) and ex vivo granzyme B (Grz B) levels (R = .50-.65, P <= .001). Following vaccination, granzyme B levels were significantly higher in the SD compared to the BD group (P <= .0002). These results suggest that SD influenza vaccine produces Th1 and CTL responses while BD may produce a Th2 response that poorly stimulates the CTL response. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3294 / 3300
页数:7
相关论文
共 29 条
[11]   Single-cell perforin and granzyme expression reveals the anatomical localization of effector CD8+ T cells in influenza virus-infected mice [J].
Johnson, BJ ;
Costelloe, EO ;
Fitzpatrick, DR ;
Haanen, JBAG ;
Schumacher, TNM ;
Brown, LE ;
Kelso, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2657-2662
[12]   Optimization of a peptide-based protocol employing IL-7 for in vitro restimulation of human cytotoxic T lymphocyte precursors [J].
Lalvani, A ;
Dong, T ;
Ogg, G ;
Pathan, AA ;
Newell, H ;
Hill, AVS ;
McMichael, AJ ;
Rowland-Jones, S .
JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 210 (01) :65-77
[13]   Granzyme B: a natural born killer [J].
Lord, SJ ;
Rajotte, RV ;
Korbutt, GS ;
Bleackley, RC .
IMMUNOLOGICAL REVIEWS, 2003, 193 (01) :31-38
[14]   INFLUENZA SUBUNIT VACCINE - ANTIBODY-RESPONSES TO ONE AND 2 DOSES OF VACCINE AND LENGTH OF RESPONSE, WITH PARTICULAR REFERENCE TO ELDERLY [J].
MACKENZIE, JS .
BMJ-BRITISH MEDICAL JOURNAL, 1977, 1 (6055) :200-202
[15]   Effect of congestive heart failure on humoral and ex vivo cellular immune responses to influenza vaccination in older adults [J].
McElhaney, JE ;
Herre, JM ;
Lawson, ML ;
Cole, SK ;
Burke, BL ;
Hooton, JW .
VACCINE, 2004, 22 (5-6) :681-688
[16]   The cell-mediated cytotoxic response to influenza vaccination using an assay for granzyme B activity [J].
McElhaney, JE ;
Pinkoski, MJ ;
Upshaw, CM ;
Bleackley, RC .
JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 190 (01) :11-20
[17]   Granzyme B: a marker of risk for influenza in institutionalized older adults [J].
McElhaney, JE ;
Gravenstein, S ;
Upshaw, CM ;
Hooton, JW ;
Krause, P ;
Drinka, P ;
Bleackley, RC .
VACCINE, 2001, 19 (27) :3744-3751
[18]   Responses to influenza vaccination in different T-cell subsets: a comparison of healthy young and older adults [J].
McElhaney, JE ;
Upshaw, CM ;
Hooton, JW ;
Lechelt, KE ;
Meneilly, GS .
VACCINE, 1998, 16 (18) :1742-1747
[19]   Role of humoral and cell-mediated immunity in protection from influenza disease after immunization of healthy elderly [J].
Murasko, DM ;
Bernstein, ED ;
Gardner, EM ;
Gross, P ;
Munk, G ;
Dran, S ;
Abrutyn, E .
EXPERIMENTAL GERONTOLOGY, 2002, 37 (2-3) :427-439
[20]   THE EFFICACY AND COST-EFFECTIVENESS OF VACCINATION AGAINST INFLUENZA AMONG ELDERLY PERSONS LIVING IN THE COMMUNITY [J].
NICHOL, KL ;
MARGOLIS, KL ;
WUORENMA, J ;
VONSTERNBERG, T .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (12) :778-784