Be more specific! Laser-assisted microdissection of plant cells

被引:95
作者
Day, RC
Grossniklaus, U
Macknight, RC
机构
[1] Univ Otago, Dept Biochem, Dunedin, New Zealand
[2] Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland
[3] Univ Zurich, Zurich Basel Plant Sci Ctr, CH-8008 Zurich, Switzerland
关键词
D O I
10.1016/j.tplants.2005.06.006
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Laser-assisted microdissection (LAM) is a powerful tool for isolating specific tissues, cell types and even organelles from sectioned biological specimen in a manner conducive to the extraction of RNA, DNA or protein. LAM, which is an established technique in many areas of biology, has now been successfully adapted for use with plant tissues. Here, we provide an overview of the processes involved in conducting a successful LAM study in plants and review recent developments that have made this technique even more desirable. We also discuss how the technology might be exploited to answer some pertinent questions in plant biology.
引用
收藏
页码:397 / 406
页数:10
相关论文
共 90 条
  • [1] Application of differential display to immunological research
    Ali, M
    Markham, AF
    Isaacs, JD
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2001, 250 (1-2) : 29 - 43
  • [2] A faithful method for PCR-mediated global mRNA amplification and its integration into microarray analysis on laser-captured cells
    Aoyagi, K
    Tatsuta, T
    Nishigaki, M
    Akimoto, S
    Tanabe, C
    Omoto, Y
    Hayashi, S
    Sakamoto, H
    Sakamoto, M
    Yoshida, T
    Terada, M
    Sasaki, H
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 300 (04) : 915 - 920
  • [3] Functional genomics of cell elongation in developing cotton fibers
    Arpat, AB
    Waugh, M
    Sullivan, JP
    Gonzales, M
    Frisch, D
    Main, D
    Wood, T
    Leslie, A
    Wing, RA
    Wilkins, TA
    [J]. PLANT MOLECULAR BIOLOGY, 2004, 54 (06) : 911 - 929
  • [4] Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem
    Asano, T
    Masumura, T
    Kusano, H
    Kikuchi, S
    Kurita, A
    Shimada, H
    Kadowaki, K
    [J]. PLANT JOURNAL, 2002, 32 (03) : 401 - 408
  • [5] Protein profiling of complete mole and normal placenta using ProteinChip analysis on laser capture microdissected cells
    Batorfi, J
    Ye, B
    Mok, SC
    Cseh, I
    Berkowitz, RS
    Fulop, V
    [J]. GYNECOLOGIC ONCOLOGY, 2003, 88 (03) : 424 - 428
  • [6] Quantitative analysis of mRNA amplification by in vitro transcription
    Baugh, L. R.
    Hill, A. A.
    Brown, E. L.
    Hunter, Craig P.
    [J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (05)
  • [7] Laser pressure catapulting (LPC): Optimization LPC-system and genotyping of colorectal carcinomas
    Bazan, V
    La Rocca, G
    Corsale, S
    Agnese, V
    Macaluso, M
    Migliavacca, M
    Gregorio, V
    Cascio, S
    Sisto, PS
    Di Fede, G
    Buscemi, M
    Fiorentino, E
    Passantino, R
    Morello, V
    Tomasino, RM
    Russo, A
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2005, 202 (02) : 503 - 509
  • [8] A gene expression map of the Arabidopsis root
    Birnbaum, K
    Shasha, DE
    Wang, JY
    Jung, JW
    Lambert, GM
    Galbraith, DW
    Benfey, PN
    [J]. SCIENCE, 2003, 302 (5652) : 1956 - 1960
  • [9] New aspects of laser microdissection in research and routine
    Burgemeister, R
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2005, 53 (03) : 409 - 412
  • [10] Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis
    Casson, S
    Spencer, M
    Walker, K
    Lindsey, K
    [J]. PLANT JOURNAL, 2005, 42 (01) : 111 - 123