Cross-reactivity within the profilin panallergen family investigated by comparison of recombinant profilins from pear (Pyr c 4), cherry (Pru av 4) and celery (Api g 4) with birch pollen profilin Bet v 2

被引:80
作者
Scheurer, S
Wangorsch, A
Nerkamp, J
Skov, PS
Ballmer-Weber, B
Wüthrich, B
Haustein, D
Vieths, S
机构
[1] Paul Ehrlich Inst, Dept Allergol, D-63225 Langen, Germany
[2] Univ Bayreuth, Bayreuth, Germany
[3] RefLab, Copenhagen, Denmark
[4] Natl Univ Hosp, Zurich, Switzerland
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2001年 / 756卷 / 1-2期
关键词
food allergy; cross reactivity; profilin; Pru av 4; Pyr c 4; Api g 4; Bet v 2;
D O I
10.1016/S0378-4347(01)00090-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Profilin is a panallergen which is recognised by IgE From about 20% of birch pollen- and plant food-allergic patients. Little is known about epitope diversity among these homologous proteins, and about the correlation between IeE-cross-reactivity and allergenic reactivity. Plant food profilins from pear (Pyr c 4) and cherry (Pru av 4) were cloned by polymerase chain reaction and produced in Escherichia coli BL21. The profilins were purified as non-fusion proteins by affinity chromatography on poly-(L-proline)-Sepharose and characterized by immunoblotting, IgE-inhibition experiments and histamine release assays. The coding regions of the cDNA of pear and cherry profilin were identified as a 393 bp open reading frame. The deduced amino acid sequences showed high identities with birch pollen profilin Bet v 2 (76-83%) and other allergenic plant profilins. Pyr c 4 and Pru av 4 were investigated for their immunological properties in comparison with profilins from celery (Api g 4) and birch pollen (Bet v 2). Forty-three of 49 patients (88%), preselected for an IgE-reactivity with Bet v 2 showed specific IgE-antibodies to the recombinant pear protein, 92% of the sera were positive with the recombinant cherry allergen and 80% of the sera were reactive with the celery protein. Inhibition experiments showed a strong cross-reactivity of IgE with profilins from plant food and birch pollen. However, IgE binding profiles also indicated the presence of epitope differences among related profilins. All investigated profilins, Pyr c 4, Pru av 4, Api g 4 and Bet v 2, presented almost identical allergenic properties in cellular mediator release tests. Therefore, cross-reactivities between related profilins may explain pollen-related allergy to food in a minority of patients. The nucleotide sequences reported have been submitted to the Genbank database under accession numbers AF129424 (Pyr c 4) and AF129425 (Pru av 4). (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:315 / 325
页数:11
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