Cross-reactivity within the profilin panallergen family investigated by comparison of recombinant profilins from pear (Pyr c 4), cherry (Pru av 4) and celery (Api g 4) with birch pollen profilin Bet v 2

被引:80
作者
Scheurer, S
Wangorsch, A
Nerkamp, J
Skov, PS
Ballmer-Weber, B
Wüthrich, B
Haustein, D
Vieths, S
机构
[1] Paul Ehrlich Inst, Dept Allergol, D-63225 Langen, Germany
[2] Univ Bayreuth, Bayreuth, Germany
[3] RefLab, Copenhagen, Denmark
[4] Natl Univ Hosp, Zurich, Switzerland
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2001年 / 756卷 / 1-2期
关键词
food allergy; cross reactivity; profilin; Pru av 4; Pyr c 4; Api g 4; Bet v 2;
D O I
10.1016/S0378-4347(01)00090-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Profilin is a panallergen which is recognised by IgE From about 20% of birch pollen- and plant food-allergic patients. Little is known about epitope diversity among these homologous proteins, and about the correlation between IeE-cross-reactivity and allergenic reactivity. Plant food profilins from pear (Pyr c 4) and cherry (Pru av 4) were cloned by polymerase chain reaction and produced in Escherichia coli BL21. The profilins were purified as non-fusion proteins by affinity chromatography on poly-(L-proline)-Sepharose and characterized by immunoblotting, IgE-inhibition experiments and histamine release assays. The coding regions of the cDNA of pear and cherry profilin were identified as a 393 bp open reading frame. The deduced amino acid sequences showed high identities with birch pollen profilin Bet v 2 (76-83%) and other allergenic plant profilins. Pyr c 4 and Pru av 4 were investigated for their immunological properties in comparison with profilins from celery (Api g 4) and birch pollen (Bet v 2). Forty-three of 49 patients (88%), preselected for an IgE-reactivity with Bet v 2 showed specific IgE-antibodies to the recombinant pear protein, 92% of the sera were positive with the recombinant cherry allergen and 80% of the sera were reactive with the celery protein. Inhibition experiments showed a strong cross-reactivity of IgE with profilins from plant food and birch pollen. However, IgE binding profiles also indicated the presence of epitope differences among related profilins. All investigated profilins, Pyr c 4, Pru av 4, Api g 4 and Bet v 2, presented almost identical allergenic properties in cellular mediator release tests. Therefore, cross-reactivities between related profilins may explain pollen-related allergy to food in a minority of patients. The nucleotide sequences reported have been submitted to the Genbank database under accession numbers AF129424 (Pyr c 4) and AF129425 (Pru av 4). (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:315 / 325
页数:11
相关论文
共 40 条
[31]   PROFILINS CONSTITUTE A NOVEL FAMILY OF FUNCTIONAL-PLANT PAN-ALLERGENS [J].
VALENTA, R ;
DUCHENE, M ;
EBNER, C ;
VALENT, P ;
SILLABER, C ;
DEVILLER, P ;
FERREIRA, F ;
TEJKL, M ;
EDELMANN, H ;
KRAFT, D ;
SCHEINER, O .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 175 (02) :377-385
[32]   IDENTIFICATION OF PROFILIN AS A NOVEL POLLEN ALLERGEN - IGE AUTOREACTIVITY IN SENSITIZED INDIVIDUALS [J].
VALENTA, R ;
DUCHENE, M ;
PETTENBURGER, K ;
SILLABER, C ;
VALENT, P ;
BETTELHEIM, P ;
BREITENBACH, M ;
RUMPOLD, H ;
KRAFT, D ;
SCHEINER, O .
SCIENCE, 1991, 253 (5019) :557-560
[33]   PURIFICATION AND CHARACTERIZATION OF AN ALLERGEN FROM CELERY IMMUNOCHEMICALLY RELATED TO AN ALLERGEN PRESENT IN SEVERAL OTHER PLANT-SPECIES - IDENTIFICATION AS A PROFILIN [J].
VALLIER, P ;
DECHAMP, C ;
VALENTA, R ;
VIAL, O ;
DEVILLER, P .
CLINICAL AND EXPERIMENTAL ALLERGY, 1992, 22 (08) :774-782
[34]   Characterization of recombinant Mercurialis annua major allergen Mer a 1 (profilin) [J].
Vallverdú, A ;
Asturias, JA ;
Arilla, MC ;
Gómez-Bayón, N ;
Martínez, A ;
Martínez, J ;
Palacios, R .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1998, 101 (03) :363-370
[35]   POLLEN-RELATED ALLERGY TO PEACH AND APPLE - AN IMPORTANT ROLE FOR PROFILIN [J].
VANREE, R ;
FERNANDEZRIVAS, M ;
CUEVAS, M ;
VANWIJNGAARDEN, M ;
AALBERSE, RC .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1995, 95 (03) :726-734
[36]   PROFILIN IS A CROSS-REACTIVE ALLERGEN IN POLLEN AND VEGETABLE FOODS [J].
VANREE, R ;
VOITENKO, V ;
VANLEEUWEN, WA ;
AALBERSE, RC .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1992, 98 (02) :97-104
[37]   CHARACTERIZATION OF THE 18-KDA APPLE ALLERGEN BY 2-DIMENSIONAL IMMUNOBLOTTING AND MICROSEQUENCING [J].
VIETHS, S ;
SCHONING, B ;
PETERSEN, A .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1994, 104 (04) :399-404
[38]  
VIETHS S, 1995, ANN ALLERG ASTHMA IM, V75, P48
[39]   High-level expression in Escherichia coli and purification of recombinant plant profilins: Comparison of IgE-binding capacity and allergenic activity [J].
Vrtala, S ;
Wiedemann, P ;
Mittermann, I ;
Eichler, HG ;
Sperr, WR ;
Valent, P ;
Kraft, D ;
Valenta, R .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 226 (01) :42-50
[40]   CELERY ALLERGY ASSOCIATED WITH BIRCH AND MUGWORT POLLINOSIS [J].
WUTHRICH, B ;
STAGER, J ;
JOHANSSON, SGO .
ALLERGY, 1990, 45 (08) :566-571