Rv0216, a conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdog fold

被引：22

作者：

Castell, A ^{[1
]}

Johansson, P ^{[1
]}

Unge, T ^{[1
]}

Jones, TA ^{[1
]}

Bäckbo, K ^{[1
]}

机构：

[1] Uppsala Univ, Ctr Biomed, Dept Cell & Mol Biol, SE-75124 Uppsala, Sweden

来源：

PROTEIN SCIENCE | 2005年 / 14卷 / 07期

关键词：

Mycobacterium tuberculosis; Rv0216; hotdog fold; protein structure; crystallography;

D O I：

10.1110/ps.051442305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Mycobacterium tuberculosis genome contains about 4000 genes, of which approximately a third code for proteins of unknown function or are classified as conserved hypothetical proteins. We have determined the three-dimensional structure of one of these, the rv0216 gene product, which has been shown to be essential for M. tuberculosis growth in vivo. The structure exhibits the greatest similarity to bacterial and eukaryotic hydratases that catalyse the R-specific hydration of 2-enoyl coenzyme A. However, only part of the catalytic machinery is conserved in Rv0216 and it showed no activity for the substrate crotonyl-CoA. The structure of Rv0216 allows us to assign new functional annotations to a family of seven other M. tuberculosis proteins, a number if which are essential for bacterial survival during infection and growth.

引用

页码：1850 / 1862

页数：13

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