Dry reaction of proteins with carbohydrates at 120°C yields neoglycoconjugates

被引：17

作者：

Boratynski, J ^{[1
]}

机构：

[1] Polish Acad Sci, Ludwik Hirszfeld Inst Immunol & Expt Therapy, Dept Tumor Immunol, PL-53114 Wroclaw, Poland

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 09期

关键词：

D O I：

10.1023/A:1008821205834

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lyophilized mixture of reducing sugar (lactose) and bovine serum albumin (BSA) heated to a temperature of above 100 degrees C gives neoglycoconjugates. Lyophilization of water solution gives approximately 20 mol lactose/mol BSA. In the presence of NaCl or dimethylformamide the substitution is about twice as high. Reaction of glycation is pH dependent. Glycoconjugates are important tools as components of vaccines, diagnostic tests and as drug and gene carriers.

引用

页码：707 / 710

页数：4

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