Nanodissection of single- and double-stranded DNA by atomic force microscopy

被引：15

作者：

An, HJ

Guo, YC

Zhang, XD

Zhang, Y

Hu, J ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Shanghai 200030, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2005年 / 5卷 / 10期

关键词：

nanodissection; single-stranded DNA (ssDNA); double-stranded DNA (dsDNA); atomic force microscopy (AFM);

D O I：

10.1166/jnn.2005.162

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanodissection of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) has been investigated by atomic force microscopy (AFM). It is found that both ss- and dsDNA can be repeatedly dissected by an AFM tip. However, a comparison study indicates that ssDNA is a little bit more easily broken by the AFM tip than dsDNA. This is supported by the fact that the time requested to break ssDNA is shorter than that of dsDNA in the same dissection procedure under the same load. Our experiment also shows that dissection of the DNA strand is very sensitive to the load applied, and a small change of the load lead to different results.

引用

页码：1656 / 1659

页数：4

共 51 条

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