Enantioseparation of dihydrofurocoumarin derivatives by various separation modes of capillary electrophoresis

被引：12

作者：

Egger, MD ^{[1
]}

Liu, Y ^{[1
]}

Sevcík, J ^{[1
]}

Tesarová, E ^{[1
]}

Rozhkov, R ^{[1
]}

Larock, RC ^{[1
]}

Armstrong, DW ^{[1
]}

机构：

[1] Iowa State Univ, Dept Chem, Ames, IA 50011 USA

来源：

ELECTROPHORESIS | 2003年 / 24卷 / 15期

关键词：

chiral capillary electrophoresis; coumarin; cyclodextrin; enantioseparation; micelle; reversed electrode polarity; sodium doclecyl sulfate;

D O I：

10.1002/elps.200305511

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chiral dihydrofurocoumarin compounds are currently the focus of industrial and pharmacological research. These derivatives have been shown to possess many physiological properties that could be medically beneficial. This work proposes four different chiral separation methods using capillary electrophoresis and micellar capillary electrophoresis (MCE). Several different cyclodextrin chiral selectors were examined to evaluate their effectiveness in the enantioseparation of dihydrofurocoumarins. In addition, the effects of the chiral selector concentration, the presence of an organic modifier, run buffer pH, and in two cases, the ratio between the chiral selector and an additional charged pseudo-phase were investigated. Overall, the best separations for this class of chiral compounds were achieved using sulfated beta-cyclodextrins at low pH in the reversed polarity mode.

引用

页码：2650 / 2656

页数：7

共 28 条

[1]

*ADV SEP TECHN INC, 2001, CHIR HDB

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