MicroRNA microarrays on archive bone marrow core biopsies of leukemias-Method validation

被引：17

作者：

Borze, Ioana ^{[1
]}

Guled, Mohamed ^{[1
]}

Musse, Suad ^{[1
]}

Raunio, Anna ^{[1
,2
]}

Elonen, Erkki ^{[3
]}

Saarinen-Pihkala, Ulla ^{[4
]}

Karjalainen-Lindsberg, Marja-Liisa ^{[2
]}

Lahti, Leo ^{[1
,5
]}

Knuutila, Sakari ^{[1
]}

机构：

[1] Univ Helsinki, Haartman Inst, Dept Pathol, FI-00014 Helsinki, Finland

[2] Univ Helsinki, HUSLAB, Cent Hosp, Dept Pathol,Cent Lab Pathol, FI-00014 Helsinki, Finland

[3] Univ Helsinki, Cent Hosp, Dept Hematol, FI-00014 Helsinki, Finland

[4] Univ Helsinki, Hosp Children & Adolescents, FI-00014 Helsinki, Finland

[5] Aalto Univ, Sch Sci & Technol, Dept Informat & Comp Sci, Espoo, Finland

来源：

LEUKEMIA RESEARCH | 2011年 / 35卷 / 02期

关键词：

Bone marrow core biopsy; Bone marrow aspirate; miRNA profiling; ALL; CML; ACUTE LYMPHOBLASTIC-LEUKEMIA; PARAFFIN-EMBEDDED TISSUES; EXPRESSION PROFILES; CD34(+) CELLS; MIRNAS; CANCER; MALIGNANCIES; PATHOLOGY; SAMPLES; FROZEN;

D O I：

10.1016/j.leukres.2010.08.005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Due to availability of bone marrow core biopsies (CB) in many pathology laboratories, we evaluated the quality and the biological information of the miRNA profiling using 9 acute lymphoblastic leukemia (ALL) and 9 chronic myeloid leukemia (CML) matched CB and bone marrow aspirates (BA). Technical replicates showed reproducible results across platforms and clustered together in hierarchical clustering analysis; and matched samples showed similar biological content having common differentially expressed miRNAs against the same control samples. We showed, that CBs, which have underwent decalcification in addition to formalin-fixation, are suitable for miRNA profiling. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：188 / 195

页数：8

共 33 条

[1] Down-Regulation of hsa-miR-10a in Chronic Myeloid Leukemia CD34+ Cells Increases USF2-Mediated Cell Growth [J].

Agirre, Xabier ;

Jimenez-Velasco, Antonio ;

San Jose-Eneriz, Edurne ;

Garate, Leire ;

Bandres, Eva ;

Cordeu, Lucia ;

Aparicio, Oscar ;

Saez, Borja ;

Navarro, German ;

Vilas-Zornoza, Amaia ;

Perez-Roger, Ignacio ;

Garcia-Foncillas, Jesus ;

Torres, Antonio ;

Heiniger, Anabel ;

Jose Calasanz, Maria ;

Fortes, Puri ;

Roman-Gomez, Jose ;

Prosper, Felipe .

MOLECULAR CANCER RESEARCH, 2008, 6 (12) :1830-1840

[2] miR-15a and miR-16-1 in cancer: discovery, function and future perspectives [J].

Aqeilan, R. I. ;

Calin, G. A. ;

Croce, C. M. .

CELL DEATH AND DIFFERENTIATION, 2010, 17 (02) :215-220

[3] Potential therapeutic applications of miRNA-based technology in hematological malignancies [J].

Barbarotto, Elisa ;

Calin, George A. .

CURRENT PHARMACEUTICAL DESIGN, 2008, 14 (21) :2040-2050

[4] Oligoarray comparative genomic hybridization in polycythemia vera and essential thrombocythemia [J].

Borze, Ioana ;

Mustjokio, Satu ;

Juvonen, Eeva ;

Knuutila, Sakari .

HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, 2008, 93 (07) :1098-1100

[5] Genetic and epigenetic silencing of microRNA-203 enhances ABL1 and BCR-ABL1 oncogene expression [J].

Bueno, Maria J. ;

Perez de Castro, Ignacio ;

de Cedron, Marta Gomez ;

Santos, Javier ;

Calin, George A. ;

Cigudosa, Juan C. ;

Croce, Carlo M. ;

Fernandez-Piqueras, Jose ;

Malumbres, Marcos .

CANCER CELL, 2008, 13 (06) :496-506

[6] Causes and consequences of microRNA dysregulation in cancer [J].

Croce, Carlo M. .

NATURE REVIEWS GENETICS, 2009, 10 (10) :704-714

[7] Evaluation and validation of total RNA extraction methods for MicroRNA expression analyses in formalin-fixed, paraffin-embedded tissues [J].

Doleshal, Martina ;

Magotra, Amber A. ;

Choudhury, Bhavna ;

Cannon, Brian D. ;

Labourier, Emmanuel ;

Szafranska, Anna E. .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2008, 10 (03) :203-211

[8] Microarray analysis of RNA extracted from formalin-fixed, paraffin-embedded and matched fresh-frozen ovarian adenocarcinomas [J].

Fedorowicz, Grazyna ;

Guerrero, Steve ;

Wu, Thomas D. ;

Modrusan, Zora .

BMC MEDICAL GENOMICS, 2009, 2

[9] Ancillary techniques in bone marrow pathology: molecular diagnostics on bone marrow trephine biopsies [J].

Fend, F ;

Bock, O ;

Kremer, M ;

Specht, K ;

Quintanilla-Martinez, L .

VIRCHOWS ARCHIV, 2005, 447 (06) :909-919

[10] Characterization of B- and T-Lineage Acute Lymphoblastic Leukemia by Integrated Analysis of MicroRNA and mRNA Expression Profiles [J].

Fulci, Valerio ;

Colombo, Teresa ;

Chiaretti, Sabina ;

Messina, Monica ;

Citarella, Franca ;

Tavolaro, Simona ;

Guarini, Anna ;

Foa, Robin ;

Macino, Giuseppe .

GENES CHROMOSOMES & CANCER, 2009, 48 (12) :1069-1082

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