Proanthocyanidin from grape seeds inactivates the PI3-kinase/PKB pathway and induces apoptosis in a colon cancer cell line

被引：131

作者：

Engelbrecht, A. -M. ^{[1
]}

Mattheyse, M. ^{[1
]}

Ellis, B. ^{[1
]}

Loos, B. ^{[1
]}

Thomas, M. ^{[1
]}

Smith, R. ^{[1
]}

Peters, S. ^{[1
]}

Smith, C. ^{[1
]}

Myburgh, K. ^{[1
]}

机构：

[1] Univ Stellenbosch, Dept Physiol Sci, ZA-7600 Stellenbosch, South Africa

来源：

CANCER LETTERS | 2007年 / 258卷 / 01期

关键词：

PI3-k; PKB/Akt; apoptosis; colon cancer;

D O I：

10.1016/j.canlet.2007.08.020

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of this investigation was to evaluate the chemopreventative/antiproliferative potential of a grape seed proanthocyanidin extract (GSPE) against colon cancer cells (CaCo2 cells) and to investigate its mechanism of action. GSPE (10-100 mu g/ml) significantly inhibited cell viability and increased apoptosis in CaCo2 cells, but did not alter viability in the normal colon cell line (NCM460). The increased apoptosis observed in GSPE-treated CaCo2 cells correlated with an attenuation of PI3-kinase (p 110 and p85 subunits) and decreased PKB Ser 473 phosphorylation. GSPE might thus exert its beneficial effects by means of increased apoptosis and suppression of the important PI3-kinase survival-related pathway. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：144 / 153

页数：10

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