Analysis of antioxidant compounds in sweet orange peel by HPLC-diode array detection-electrospray ionization mass spectrometry

被引:117
作者
Anagnostopoulou, MA
Kefalas, P
Kokkalou, E
Assimopoulou, AN
Papageorgiou, VP
机构
[1] Aristotle Univ Thessaloniki, Fac Engn, Dept Chem Engn, Thessaloniki 54124, Greece
[2] Mediterranean Agron Inst Chania, Khania 73100, Crete, Greece
[3] Aristotle Univ Thessaloniki, Fac Med, Dept Pharm, GR-54006 Thessaloniki, Greece
关键词
HPLC-DAD-ESIMS; navel sweet orange peel; flavonoids C-glycosylated flavones;
D O I
10.1002/bmc.430
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
HPLC-diode array detection-electrospray ionization mass spectrometry was used to determine qualitatively and quantitatively the flavonoid content of several fractions and residues of extracts of Greek navel sweet orange peel (Citrus sinensis) from the region of southern Greece (Leonidi-Tripoli). The main groups of flavonoids found according to HPLC retention times, spectral data and literature references were polymethoxylated flavones, C-glycosylated flavones, O-glycosylated flavones, O-glycosylated flavanones, flavonols and phenolic acids and their derivatives. The ethyl acetate fraction which has been shown in previous work to possess the best radical scavenging activity among the others was found to contain C-glycosylated flavones, polymethoxylated flavones, O-glycosylated flavones, O-glycosylated flavanones, two phenolic acid derivatives and two unknown compounds, all in low concentrations. The group of C-glycosylated flavones was reported for the first time in the peel of Navel sweet orange. The C-glycosylated flavones found according to their spectral characteristics and literature were 6-C-beta-glucosyldiosmin, 6,8-di-C-glucopyranosylapigenin, 6,8-di-C-beta-glucosyldiosmin and two unknown. The results suggest that the ethyl acetate fraction of navel Citrus sinensis peel consists of significant antioxidant compounds and can be used as a food additive of natural origin or a pharmaceutical supplement using as a source of peel the byproducts of the orange juice industry. Copyright (c) 2004 John Wiley & Sons, Ltd.
引用
收藏
页码:138 / 148
页数:11
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