Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila

被引：234

作者：

Jiang, F

Ye, XC

Liu, X

Fincher, L

McKearin, D

Liu, QH ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Biochem, Dallas, TX 75390 USA

[2] Univ Texas, SW Med Ctr, Dept Mol Biol, Dallas, TX 75390 USA

来源：

GENES & DEVELOPMENT | 2005年 / 19卷 / 14期

关键词：

Dicer-1; R3D1-L; RNase III; dsRNA binding; pre-miRNA; miRNA;

D O I：

10.1101/gad.1334005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Drosophila melanogaster, Dicer-2/R2132 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L, enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.

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收藏

页码：1674 / 1679

页数：6

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