Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom

被引:34
作者
Deolindo, P
Teixeira-Ferreira, AS
Melo, EJT
Arnholdt, ACV
de Souza, W
Alves, EW
DaMatta, RA
机构
[1] Univ Estadual Norte Fluminense, Lab Quim & Funcao Proteinas & Peptideos, BR-28013600 Campos Dos Goytacazes, RJ, Brazil
[2] Univ Estadual Norte Fluminense, Lab Biol Celular & Tecidual, BR-28013600 Campos Dos Goytacazes, RJ, Brazil
[3] Univ Estadual Norte Fluminense, Lab Biol Reconhecer, Ctr Biociencias & Biotecnol, BR-28013600 Campos Dos Goytacazes, RJ, Brazil
[4] Univ Fed Rio de Janeiro, Lab Biol Celular Hertha Meyer, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2005年 / 100卷 / 01期
关键词
Trypanosoma cruzi; programmed cell death; Bothrops jararaca; snake venom;
D O I
10.1590/S0074-02762005000100006
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 mu g/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death.
引用
收藏
页码:33 / 38
页数:6
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