A bacterial extracellular DNA inhibits settling of motile progeny cells within a biofilm

被引:81
作者
Berne, Cecile [1 ]
Kysela, David T. [1 ]
Brun, Yves V. [1 ]
机构
[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
基金
美国国家卫生研究院;
关键词
CAULOBACTER-CRESCENTUS; AGROBACTERIUM-TUMEFACIENS; SURFACE ATTACHMENT; MICROBIAL BIOFILMS; GENOME SEQUENCE; SWARMER CELLS; HOLDFAST; ADHESION; RELEASE; ENVIRONMENT;
D O I
10.1111/j.1365-2958.2010.07267.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
P>In natural systems, bacteria form complex, surface-attached communities known as biofilms. This lifestyle presents numerous advantages compared with unattached or planktonic life, such as exchange of nutrients, protection from environmental stresses and increased tolerance to biocides. Despite such benefits, dispersal also plays an important role in escaping deteriorating environments and in successfully colonizing favourable, unoccupied habitat patches. The alpha-proteobacterium Caulobacter crescentus produces a motile swarmer cell and a sessile stalked cell at each cell division. We show here that C. crescentus extracellular DNA (eDNA) inhibits the ability of its motile cell type to settle in a biofilm. eDNA binds to the polar holdfast, an adhesive structure required for permanent surface attachment and biofilm formation, thereby inhibiting cell attachment. Because stalked cells associate tightly with the biofilm through their holdfast, we hypothesize that this novel mechanism acts on swarmer cells born in a biofilm, where eDNA can accumulate to a sufficient concentration to inhibit their ability to settle. By targeting a specific cell type in a biofilm, this mechanism modulates biofilm development and promotes dispersal without causing a potentially undesirable dissolution of the existing biofilm.
引用
收藏
页码:815 / 829
页数:15
相关论文
共 53 条
[31]   PHYSICAL AND GENETIC-CHARACTERIZATION OF SYMBIOTIC AND AUXOTROPHIC MUTANTS OF RHIZOBIUM-MELILOTI INDUCED BY TRANSPOSON TN5 MUTAGENESIS [J].
MEADE, HM ;
LONG, SR ;
RUVKUN, GB ;
BROWN, SE ;
AUSUBEL, FM .
JOURNAL OF BACTERIOLOGY, 1982, 149 (01) :114-122
[32]   CHARACTERIZATION OF THE ADHESIVE HOLDFAST OF MARINE AND FRESH-WATER CAULOBACTERS [J].
MERKER, RI ;
SMIT, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (08) :2078-2085
[33]   Motility and chemotaxis in Agrobacterium tumefaciens surface attachment and Biofilm formation [J].
Merritt, Peter M. ;
Danhorn, Thomas ;
Fuqua, Clay .
JOURNAL OF BACTERIOLOGY, 2007, 189 (22) :8005-8014
[34]   Fighting change with change: adaptive variation in an uncertain world [J].
Meyers, LA ;
Bull, JJ .
TRENDS IN ECOLOGY & EVOLUTION, 2002, 17 (12) :551-557
[35]   Bacterial interactions in biofilms [J].
Moons, Pieter ;
Michiels, Chris W. ;
Aertsen, Abram .
CRITICAL REVIEWS IN MICROBIOLOGY, 2009, 35 (03) :157-168
[36]   The sociobiology of biofilms [J].
Nadell, Carey D. ;
Xavier, Joao B. ;
Foster, Kevin R. .
FEMS MICROBIOLOGY REVIEWS, 2009, 33 (01) :206-224
[37]   FINE STRUCTURE OF 2 UNUSUAL STALKED BACTERIA [J].
PATE, JL ;
ORDAL, EJ .
JOURNAL OF CELL BIOLOGY, 1965, 27 (01) :133-&
[38]   BIOLOGICAL PROPERTIES + CLASSIFICATION OF CAULOBACTER GROUP [J].
POINDEXTER, JS .
BACTERIOLOGICAL REVIEWS, 1964, 28 (03) :231-&
[39]   Molecular control of bacterial death and lysis [J].
Rice, Kelly C. ;
Bayles, Kenneth W. .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2008, 72 (01) :85-+
[40]   β-1,3-glucan polysaccharides as novel one-dimensional hosts for DNA/RNA, conjugated polymers and nanoparticles [J].
Sakurai, K ;
Uezu, K ;
Numata, M ;
Hasegawa, T ;
Li, C ;
Kaneko, K ;
Shinkai, S .
CHEMICAL COMMUNICATIONS, 2005, (35) :4383-4398