Application of a C-peptide electrospray ionization-isotope dilution-liquid chromatography-tandem mass spectrometry measurement procedure for the evaluation of five C-peptide immunoassays for urine

被引:15
作者
Fierens, C
Stöckl, D
Baetens, D
De Leenheer, AP
Thienpont, LM
机构
[1] State Univ Ghent, Fac Farmaceut Wetenschappen, Analyt Chem Lab, B-9000 Ghent, Belgium
[2] AZ Middelheim, Klin Lab, B-2020 Antwerp, Belgium
[3] State Univ Ghent, Fac Farmaceut Wetenschappen, Lab Med Biochem & Klin Anal, B-9000 Ghent, Belgium
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 792卷 / 02期
关键词
immunoassays; split sample measurements; C-peptides;
D O I
10.1016/S1570-0232(03)00268-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This study applied electrospray ionization-isotope dilution-liquid chromatography-tandem mass spectrometry for the evaluation of five urinary C-peptide immumoassays via split-sample measurements. The immunoassays measured in duplicate in the same run, the comparison method in triplicate over different runs. From the data, the within-run imprecision and the method comparison total RSDs were calculated. Regression analysis revealed on the one hand systematic differences, on the other. an excellent correlation between the test and comparison methods. From the spread of the data around the regression line in comparison with the 95% prediction intervals from the total RSD, sample-related effects and/or specificity problems were apparent and investigated. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:249 / 259
页数:11
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