Higher-energy C-trap dissociation for peptide modification analysis

被引:715
作者
Olsen, Jesper V.
Macek, Boris
Lange, Oliver
Makarov, Alexander
Horning, Stevan
Mann, Matthias
机构
[1] Thermo Fisher Sci GmbH, D-28199 Bremen, Germany
[2] Max Planck Inst Biochem, Dept Proteom & Signal Transduc, D-82131 Martinsried, Germany
关键词
D O I
10.1038/NMETH1060
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Peptide sequencing is the basis of mass spectrometry-driven proteomics. Here we show that in the linear ion trap-orbitrap mass spectrometer (LTQ Orbitrap) peptide ions can be efficiently fragmented by high- accuracy and full-mass-range tandem mass spectrometry (MS/MS) via higher-energy C-trap dissociation (HCD). Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. Additionally we show that an added octopole collision cell facilitates de novo sequencing.
引用
收藏
页码:709 / 712
页数:4
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