Soil bacterial and fungal communities across a pH gradient in an arable soil

被引：3137

作者：

Rousk, Johannes ^{[1
]}

Baath, Erland ^{[1
]}

Brookes, Philip C. ^{[2
]}

Lauber, Christian L. ^{[3
]}

Lozupone, Catherine ^{[4
]}

Caporaso, J. Gregory ^{[4
]}

Knight, Rob ^{[4
,5
]}

Fierer, Noah ^{[3
,6
]}

机构：

[1] Lund Univ, Dept Microbial Ecol, S-22362 Lund, Sweden

[2] Rothamsted Res, Dept Soil Sci, Harpenden, Herts, England

[3] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA

[4] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

[5] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA

[6] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA

来源：

ISME JOURNAL | 2010年 / 4卷 / 10期

基金：

瑞典研究理事会; 美国安德鲁·梅隆基金会; 美国国家科学基金会;

关键词：

bacterial community; fungal community; pyrosequencing; quantitative PCR; Rothamsted Hoosfield Acid Strip; soil pH; FOREST SOILS; MICROBIAL COMMUNITIES; DIVERSITY; GROWTH; ADAPTATION; ABUNDANCE; PATTERNS; PRIMERS; SAMPLES;

D O I：

10.1038/ismej.2010.58

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Soils collected across a long-term liming experiment (pH 4.0-8.3), in which variation in factors other than pH have been minimized, were used to investigate the direct influence of pH on the abundance and composition of the two major soil microbial taxa, fungi and bacteria. We hypothesized that bacterial communities would be more strongly influenced by pH than fungal communities. To determine the relative abundance of bacteria and fungi, we used quantitative PCR (qPCR), and to analyze the composition and diversity of the bacterial and fungal communities, we used a bar-coded pyrosequencing technique. Both the relative abundance and diversity of bacteria were positively related to pH, the latter nearly doubling between pH 4 and 8. In contrast, the relative abundance of fungi was unaffected by pH and fungal diversity was only weakly related with pH. The composition of the bacterial communities was closely defined by soil pH; there was as much variability in bacterial community composition across the 180-m distance of this liming experiment as across soils collected from a wide range of biomes in North and South America, emphasizing the dominance of pH in structuring bacterial communities. The apparent direct influence of pH on bacterial community composition is probably due to the narrow pH ranges for optimal growth of bacteria. Fungal community composition was less strongly affected by pH, which is consistent with pure culture studies, demonstrating that fungi generally exhibit wider pH ranges for optimal growth. The ISME Journal (2010) 4, 1340-1351; doi: 10.1038/ismej.2010.58; published online 6 May 2010

引用

页码：1340 / 1351

页数：12