Study of streptavidin coated onto PAMAM dendrimer modified magnetite nanoparticles

被引：64

作者：

Gao, F ^{[1
]}

Pan, BF ^{[1
]}

Zheng, WM ^{[1
]}

Ao, LM ^{[1
]}

Gu, HC ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Engn Res Ctr Nano Sci & Technol, Shanghai 200030, Peoples R China

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2005年 / 293卷 / 01期

关键词：

magnetite nanoparticles (MNP); polyamidoamine; PAMAM; dendrimer; streptavidin (SA); biotin; silane; ELISA;

D O I：

10.1016/j.jmmm.2005.01.042

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Polyamidoamine dendrimer was synthesized on the surface of amino silane modified magnetite nanoparticles. After coating of streptavidin to these dendrimer-modified magnetite nanoparticles, an up to 3.4 times higher amount of streptavidin (SA) was measured compared to magnetite nanoparticles modified with only amino silane. The biotin-binding capacity of SA thus increased after dendrimer modification. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：48 / 54

页数：7

共 13 条

[1] AN IMPROVED METHOD FOR THE SINGLE-STEP PURIFICATION OF STREPTAVIDIN [J].

BAYER, EA ;

BENHUR, H ;

GITLIN, G ;

WILCHEK, M .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1986, 13 (02) :103-112

[2] Magnetic ion-exchange nano- and microparticles for medical, biochemical and molecular biological applications [J].

Bergemann, C ;

Müller-Schulte, D ;

Oster, J ;

à Brassard, L ;

Lübbe, AS .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1999, 194 (1-3) :45-52

[3] Dendrimer-encapsulated metal nanoparticles: Synthesis, characterization, and applications to catalysis [J].

Crooks, RM ;

Zhao, MQ ;

Sun, L ;

Chechik, V ;

Yeung, LK .

ACCOUNTS OF CHEMICAL RESEARCH, 2001, 34 (03) :181-190

[4] Methods to determine biotin-binding capacity of streptavidin-coated magnetic particles [J].

Dorgan, L ;

Magnotti, R ;

Hou, JM ;

Engle, T ;

Ruley, K ;

Shull, B .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1999, 194 (1-3) :69-75

[5] High sensitivity detection of molecular recognition using magnetically labelled biomolecules and magnetoresistive sensors [J].

Graham, DL ;

Ferreira, HA ;

Freitas, PP ;

Cabral, JMS .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (04) :483-488

[6]

Hawkins T., 1998, US. Patent, Patent No. [5,705,628, 5705628, 5705628(A)]

[7] Magnetorelaxometry -: a new binding specific detection method based on magnetic nanoparticles [J].

Lange, J ;

Kötitz, R ;

Haller, A ;

Trahms, L ;

Semmler, W ;

Weitschies, W .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2002, 252 (1-3) :381-383

[8] The effect of bovine serum albumin on the binding constant and stoichiometry of biocompatible magnetic fluids [J].

Macaroff, PP ;

Oliveira, DM ;

Lacava, ZGA ;

Azevedo, RB ;

Lima, ECD ;

Morais, PC ;

Tedesco, AC .

IEEE TRANSACTIONS ON MAGNETICS, 2004, 40 (04) :3027-3029

[9] Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex [J].

Matsunaga, T ;

Sato, R ;

Kamiya, S ;

Tanaka, T ;

Takeyama, H .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1999, 194 (1-3) :126-131

[10] Direct binding of protein to magnetic particles [J].

Mehta, RV ;

Upadhyay, RV ;

Charles, SW ;

Ramchand, CN .

BIOTECHNOLOGY TECHNIQUES, 1997, 11 (07) :493-496

← 1 2 →