Genomic Exploration of the Hemiascomycetous Yeasts:: 7.: Saccharomyces servazzii

The genome of Saccharomyces servazzii was analyzed with 2570 random sequence tags totalling 2.3 Mb. BLASTX comparisons revealed a minimum of 1420 putative open reading frames with significant homology to Saccharomyces cervisiae (58% aa identity on average), two with Schizosaccharomyces pombe and one with a human protein, confirming that S. servazzii is closely related to S.cerevisiae. About 25% of the S, ser,servazzii genes were identified, assuming that the gene complement is identical in both yeasts. S. servazii carries very few transposable elements related to Ty elements in S, cervisiae. Most of the mitochondrial genes were identified in eight contigs altogether spanning 25 kb for a predicted size of 29 kb, A significant match with the Kluyveromyves lactis linear DNA plasmid pGKL-1 encoded RF4 killer protein suggests that a related plasmid exists in S, scr,servazzii. The sequences have been deposited with EMBL under the accession numbers AL402279-AL404848. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.