Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma

被引：121

作者：

Nohata, Nijiro ^{[1
,2
]}

Hanazawa, Toyoyuki ^{[2
]}

Kikkawa, Naoko ^{[1
,2
]}

Mutallip, Muradil ^{[1
,2
]}

Fujmura, Lisa ^{[3
]}

Yoshino, Hirofumi ^{[4
]}

Kawakami, Kazumori ^{[4
]}

Chiyomaru, Takeshi ^{[4
]}

Enokida, Hideki ^{[4
]}

Nakagawa, Masayuki ^{[4
]}

Okamoto, Yoshitaka ^{[2
]}

Seki, Naohiko ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Funct Genom, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, Dept Otorhinolaryngol Head & Neck Surg, Chiba 2608670, Japan

[3] Chiba Univ, Biomed Res Ctr, Chiba 2608670, Japan

[4] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Urol, Kagoshima 890, Japan

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2011年 / 38卷 / 01期

关键词：

miR-133a; HNSCC; caveolin-1; migration; invasion; UP-REGULATION; MESENCHYMAL TRANSITION; MICRORNA SIGNATURES; POOR-PROGNOSIS; FREE SURVIVAL; EXPRESSION; CANCER; GROWTH; OVEREXPRESSION; IDENTIFICATION;

D O I：

10.3892/ijo_00000840

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides that can function as oncogenes or tumor suppressors in human cancer. Downregulation of the miRNA miR-133a in many type of cancers, and a reduction of cell proliferation, migration, and invasion upon over-expression, suggests that miR-133a is a tumor suppressor. In this study, genome-wide gene expression analysis of HNSCC cells that over-express miR-133a showed that caveolin-1 (CAV1), a multifunctional scaffolding protein, is down-regulated, a result that was confirmed by real-time PCR and Western blot analysis. A luciferase reporter assay revealed that miR-133a is directly bound to CAV1 mRNA. Cancer cell migration and invasion were significantly inhibited in HNSCC cells transfected with si-CAV1. Therefore, CAV1 functions as an oncogene in HNSCC. The identification of tumor suppressive miRNAs and their target genes could provide new insights into potential mechanism of HNSCC carcinogenesis.

引用

页码：209 / 217

页数：9

共 44 条

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