Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma

被引：121

作者：

Nohata, Nijiro ^{[1
,2
]}

Hanazawa, Toyoyuki ^{[2
]}

Kikkawa, Naoko ^{[1
,2
]}

Mutallip, Muradil ^{[1
,2
]}

Fujmura, Lisa ^{[3
]}

Yoshino, Hirofumi ^{[4
]}

Kawakami, Kazumori ^{[4
]}

Chiyomaru, Takeshi ^{[4
]}

Enokida, Hideki ^{[4
]}

Nakagawa, Masayuki ^{[4
]}

Okamoto, Yoshitaka ^{[2
]}

Seki, Naohiko ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Funct Genom, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, Dept Otorhinolaryngol Head & Neck Surg, Chiba 2608670, Japan

[3] Chiba Univ, Biomed Res Ctr, Chiba 2608670, Japan

[4] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Urol, Kagoshima 890, Japan

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2011年 / 38卷 / 01期

关键词：

miR-133a; HNSCC; caveolin-1; migration; invasion; UP-REGULATION; MESENCHYMAL TRANSITION; MICRORNA SIGNATURES; POOR-PROGNOSIS; FREE SURVIVAL; EXPRESSION; CANCER; GROWTH; OVEREXPRESSION; IDENTIFICATION;

D O I：

10.3892/ijo_00000840

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides that can function as oncogenes or tumor suppressors in human cancer. Downregulation of the miRNA miR-133a in many type of cancers, and a reduction of cell proliferation, migration, and invasion upon over-expression, suggests that miR-133a is a tumor suppressor. In this study, genome-wide gene expression analysis of HNSCC cells that over-express miR-133a showed that caveolin-1 (CAV1), a multifunctional scaffolding protein, is down-regulated, a result that was confirmed by real-time PCR and Western blot analysis. A luciferase reporter assay revealed that miR-133a is directly bound to CAV1 mRNA. Cancer cell migration and invasion were significantly inhibited in HNSCC cells transfected with si-CAV1. Therefore, CAV1 functions as an oncogene in HNSCC. The identification of tumor suppressive miRNAs and their target genes could provide new insights into potential mechanism of HNSCC carcinogenesis.

引用

页码：209 / 217

页数：9

共 44 条

[31] MicroRNA Profiling and Head and Neck Cancer [J].

Liu, Xiqiang ;

Chen, Zugen ;

Yu, Jinsheng ;

Xia, James ;

Zhou, Xiaofeng .

COMPARATIVE AND FUNCTIONAL GENOMICS, 2009,

[32] MicroRNA expression profiles classify human cancers [J].

Lu, J ;

Getz, G ;

Miska, EA ;

Alvarez-Saavedra, E ;

Lamb, J ;

Peck, D ;

Sweet-Cordero, A ;

Ebet, BL ;

Mak, RH ;

Ferrando, AA ;

Downing, JR ;

Jacks, T ;

Horvitz, HR ;

Golub, TR .

NATURE, 2005, 435 (7043) :834-838

[33] Epithelial to mesenchymal transition in head and neck squamous carcinoma - Association of src activation with E-cadherin down-regulation, vimentin expression, and aggressive tumor features [J].

Mandal, Mahitosh ;

Myers, Jeffery N. ;

Lippman, Scoff M. ;

Johnson, Faye M. ;

Williams, Michelle D. ;

Rayala, Suresh ;

Ohshiro, Kazufumi ;

Rosenthal, David I. ;

Weber, Randal S. ;

Gallick, Gary E. ;

El-Naggar, Adel K. .

CANCER, 2008, 112 (09) :2088-2100

[34] Caveolins, a family of scaffolding proteins for organizing "preassembled signaling complexes" at the plasma membrane" [J].

Okamoto, T ;

Schlegel, A ;

Scherer, PE ;

Lisanti, MP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (10) :5419-5422

[35] Distinct roles for miR-1 and miR-133a in the proliferation and differentiation of rhabdomyosarcoma cells [J].

Rao, Prakash K. ;

Missiaglia, Edoardo ;

Shields, Lauren ;

Hyde, Greg ;

Yuan, Bingbing ;

Shepherd, Christopher J. ;

Shipley, Janet ;

Lodish, Harvey F. .

FASEB JOURNAL, 2010, 24 (09) :3427-3437

[36] Caveolin-1 expression is down-regulated in cells transformed by the human papilloma virus in a p53-dependent manner. Replacement of caveolin-1 expression suppresses HPV-mediated cell transformation [J].

Razani, B ;

Altschuler, Y ;

Zhu, L ;

Pestell, RG ;

Mostov, KE ;

Lisanti, MP .

BIOCHEMISTRY, 2000, 39 (45) :13916-13924

[37] Caveolin-1 expression is a predictor of recurrence-free survival in pT2N0 prostate carcinoma diagnosed in Japanese patients [J].

Satoh, T ;

Yang, G ;

Egawa, S ;

Addai, J ;

Frolov, A ;

Kuwao, S ;

Timme, TL ;

Baba, S ;

Thompson, TC .

CANCER, 2003, 97 (05) :1225-1233

[38] The Galanin Signaling Cascade is a Candidate Pathway Regulating Oncogenesis in Human Squamous Cell Carcinoma [J].

Sugimoto, Takashi ;

Seki, Naohiko ;

Shimizu, Satoya ;

Kikkawa, Naoko ;

Tsukada, Jun ;

Shimada, Hideaki ;

Sasaki, Keita ;

Hanazawa, Toyoyuki ;

Okamoto, Yoshitaka ;

Hata, Akira .

GENES CHROMOSOMES & CANCER, 2009, 48 (02) :132-142

[39] MicroRNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma [J].

Szafranska, A. E. ;

Davison, T. S. ;

John, J. ;

Cannon, T. ;

Sipos, B. ;

Maghnouj, A. ;

Labourier, E. ;

Hahn, S. A. .

ONCOGENE, 2007, 26 (30) :4442-4452

[40] POTENTIAL ROLE OF MICRO-RNAS IN HEAD AND NECK TUMORIGENESIS [J].

Tran, Nham ;

O'Brien, Christopher J. ;

Clark, Jonathan ;

Rose, Barbara .

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 2010, 32 (08) :1099-1111

← 1 2 3 4 5 →