Simultaneous and accurate determination of one-bond 15N-13C′ and two-bond 1HN-13C′ dipolar couplings

被引：8

作者：

Ding, KY ^{[1
]}

Gronenborn, AM ^{[1
]}

机构：

[1] NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 38期

关键词：

D O I：

10.1021/ja035954e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Using the echo-anti-echo manipulation, the15N-1HN cross-peaks split in the E.COSY spectrum by the 13CO couplings are separated into different, distinct regions in the HSQC spectrum. From this novel E.COSY 15N-1HN HSQC spectrum, the small one-bond 15N-13C′ and two-bond 1HN-13C′ residual dipolar couplings can be extracted easily and accurately. These dipolar couplings provide a set of important long-range constraints for protein structure determination.

引用

收藏

页码：11504 / 11505

页数：2

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