The vascular endothelial growth factor mRNA contains an internal ribosome entry site

被引：72

作者：

Miller, DL

Dibbens, JA

Damert, A

Risau, W

Vadas, MA

Goodall, GJ

机构：

[1] Inst Med & Vet Sci, Hanson Ctr Canc Res, Adelaide, SA 5000, Australia

[2] Max Planck Inst Physiol & Klin Forsch, WG Kerckhoff Inst, Abt Mol Zellbiol, D-61231 Bad Neuheim, Germany

来源：

FEBS LETTERS | 1998年 / 434卷 / 03期

关键词：

vascular endothelial growth factor; translation; 5 '-untranslated region; ribosome;

D O I：

10.1016/S0014-5793(98)01025-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vascular endothelial growth factor (VEGF), an essential regulator of angiogenesis during early development as well as during the growth of solid tumours, bears an unusually large 5' untranslated region (5'-UTR) in the mRNA of over 1000 nucleotides, We found that the VEGF 5'-UTR, despite being GC-rich and containing an upstream short open reading frame, promotes efficient translation of a luciferase reporter. The VEGF 5'-UTR also allowed translation of luciferase from a dicistronic mRNA when placed between the two cistrons, demonstrating that it contains an internal ribosome entry site. Deletion analysis indicated that the IRES resides towards the 3' end of the 5'-UTR, (C) 1998 Federation of European Biochemical Societies.

引用

页码：417 / 420

页数：4

共 33 条

[11] INHIBITION OF VASCULAR ENDOTHELIAL GROWTH FACTOR-INDUCED ANGIOGENESIS SUPPRESSES TUMOR-GROWTH INVIVO [J].