Structure of the exopolysaccharide produced by Streptococcus thermophilus S3

被引:65
作者
Faber, EJ [1 ]
van den Haak, MJ [1 ]
Kamerling, JP [1 ]
Vliegenthart, JFG [1 ]
机构
[1] Univ Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, NL-3508 TB Utrecht, Netherlands
关键词
lactic acid bacteria; exopolysaccharide; Streptococcus thermophilus; structural analysis;
D O I
10.1016/S0008-6215(01)00013-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The exopolysaccharide of Streptococcus thermophilus S3, produced in skimmed milk, is composed of D-galactose and L-rhamnose in a molar ratio of 2:1. The polysaccharide contains 0.4 equiv of O-acetyl groups per repeating unit. Linkage analysis and 1D/2D NMR (H-1 and C-13) studies on native and O-deacetylated EPS together with nanoES-CID tandem mass spectrometry studies on oligosaccharides generated by a periodate oxidation protocol, show the polysaccharide to have the following structure: [GRAPHICS] (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:173 / 182
页数:10
相关论文
共 32 条
[1]  
ASPINALL GO, 1982, POLYSACCHARIDES, V1, P81
[2]   C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES [J].
BOCK, K ;
PEDERSEN, C .
ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1983, 41 :27-66
[3]  
Brull LP, 1998, RAPID COMMUN MASS SP, V12, P1520
[4]   Structural characterisation of the exocellular polysaccharide produced by Streptococcus thermophilus OR 901 [J].
Bubb, WA ;
Urashima, T ;
Fujiwara, R ;
Shinnai, T ;
Ariga, H .
CARBOHYDRATE RESEARCH, 1997, 301 (1-2) :41-50
[6]   A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].
CIUCANU, I ;
KEREK, F .
CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217
[7]   STRUCTURE OF AN EXOCELLULAR POLYSACCHARIDE PRODUCED BY STREPTOCOCCUS-THERMOPHILUS [J].
DOCO, T ;
WIERUSZESKI, JM ;
FOURNET, B ;
CARCANO, D ;
RAMOS, P ;
LOONES, A .
CARBOHYDRATE RESEARCH, 1990, 198 (02) :313-321
[8]   A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES [J].
DOMON, B ;
COSTELLO, CE .
GLYCOCONJUGATE JOURNAL, 1988, 5 (04) :397-409
[9]   The exopolysaccharides produced by Streptococcus thermophilus Rs and Sts have the same repeating unit but differ in viscosity of their milk cultures [J].
Faber, EJ ;
Zoon, P ;
Kamerling, JP ;
Vliegenthart, JFG .
CARBOHYDRATE RESEARCH, 1998, 310 (04) :269-276
[10]   DETERMINATION OF D AND L CONFIGURATION OF NEUTRAL MONOSACCHARIDES BY HIGH-RESOLUTION CAPILLARY GLC [J].
GERWIG, GJ ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
CARBOHYDRATE RESEARCH, 1978, 62 (02) :349-357