Structure of the exopolysaccharide produced by Streptococcus thermophilus S3

被引:65
作者
Faber, EJ [1 ]
van den Haak, MJ [1 ]
Kamerling, JP [1 ]
Vliegenthart, JFG [1 ]
机构
[1] Univ Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, NL-3508 TB Utrecht, Netherlands
关键词
lactic acid bacteria; exopolysaccharide; Streptococcus thermophilus; structural analysis;
D O I
10.1016/S0008-6215(01)00013-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The exopolysaccharide of Streptococcus thermophilus S3, produced in skimmed milk, is composed of D-galactose and L-rhamnose in a molar ratio of 2:1. The polysaccharide contains 0.4 equiv of O-acetyl groups per repeating unit. Linkage analysis and 1D/2D NMR (H-1 and C-13) studies on native and O-deacetylated EPS together with nanoES-CID tandem mass spectrometry studies on oligosaccharides generated by a periodate oxidation protocol, show the polysaccharide to have the following structure: [GRAPHICS] (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:173 / 182
页数:10
相关论文
共 32 条
[11]  
GERWIG GJ, 1979, CARBOHYD RES, V77, P1, DOI 10.1016/S0008-6215(00)83788-X
[12]   STRUCTURAL CHARACTERIZATION OF THE EXOPOLYSACCHARIDE PRODUCED BY LACTOBACILLUS-DELBRUCKII SUBSPECIES BULGARICUS RR GROWN IN SKIMMED-MILK [J].
GRUTER, M ;
LEEFLANG, BR ;
KUIPER, J ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
CARBOHYDRATE RESEARCH, 1993, 239 :209-226
[13]   STRUCTURE OF THE EXOPOLYSACCHARIDE PRODUCED BY LACTOCOCCUS-LACTIS SUBSPECIES CREMORIS H414 GROWN IN A DEFINED MEDIUM OR SKIMMED-MILK [J].
GRUTER, M ;
LEEFLANG, BR ;
KUIPER, J ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
CARBOHYDRATE RESEARCH, 1992, 231 :273-291
[14]   THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS [J].
HARD, K ;
VANZADELHOFF, G ;
MOONEN, P ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (03) :895-915
[15]  
Jansson P.E., 1976, Chem. Commun, V8, P1
[16]  
Kamerling J. P., 1989, CLIN BIOCH PRINCIPLE, V1, P176
[17]   STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE CHAIN OF KLEBSIELLA-PNEUMONIAE O1K2 (NCTC-5055) LIPOPOLYSACCHARIDE - A COMPLEMENTARY ELUCIDATION [J].
KOL, O ;
WIERUSZESKI, JM ;
STRECKER, G ;
FOURNET, B ;
ZALISZ, R ;
SMETS, P .
CARBOHYDRATE RESEARCH, 1992, 236 :339-344
[18]   Structural characterization of the exocellular polysaccharides produced by Streptococcus thermophilus SFi39 and SFi12 [J].
Lemoine, J ;
Chirat, F ;
Wieruszeski, JM ;
Strecker, G ;
Favre, N ;
Neeser, JR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (09) :3512-3518
[19]   STRUCTURE OF THE EXTRACELLULAR POLYSACCHARIDE FROM SLIME-FORMING LACTOCOCCUS-LACTIS SUBSP CREMORIS SBT-0495 [J].
NAKAJIMA, H ;
HIROTA, T ;
TOBA, T ;
ITOH, T ;
ADACHI, S .
CARBOHYDRATE RESEARCH, 1992, 224 :245-253
[20]   Structural characterization of the exopolysaccharide produced by Lactobacillus acidophilus LMG9433 [J].
Robijn, GW ;
Gallego, RG ;
vandenBerg, DJC ;
Haas, H ;
Kamerling, JP ;
Vliegenthart, JFG .
CARBOHYDRATE RESEARCH, 1996, 288 :203-218