Flow cytometric monitoring of rhodamine 123 and a cyanine dye uptake by yeast during cider fermentation

被引:29
作者
Lloyd, D [1 ]
Moran, CA [1 ]
Suller, MTE [1 ]
Dinsdale, MG [1 ]
Hayes, AJ [1 ]
机构
[1] UNIV WALES COLL CARDIFF,SCH MOLEC & MED BIOSCI,ANAT UNIT,CARDIFF CF1 3YE,S GLAM,WALES
关键词
flow cytometry; confocal laser scanning microscopy; yeast vitality; mitochondrial membrane potential; rhodamine; 123; cyanine dye;
D O I
10.1002/j.2050-0416.1996.tb00910.x
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Flow cytometry of rhodamine 123- or cyanine-stained cider yeast shows that the capacity for mitochondrial uptake of these cationic dyes is lost early in the fermentation. Survival of prolonged anaerobiosis (for at least 22 days) at high ethanol concentrations (at least 11% v/v) during cider fermentation does not require the maintenance of a measurable inner mitochondrial transmembrane electrochemical potential. Confocal laser scanning microscopy of yeasts after exposure to either of the cationic dyes confirms the lack of mitochondrial development and inability of the fermentative organisms to take up the fluorophores. Aeration of samples taken from the fermentation vessels restores the ultrastructure and the dye uptake capacity of the yeasts. This indicates that the changes are reversible, and that the organisms have retained their viability.
引用
收藏
页码:251 / 259
页数:9
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