High-density SNP haplotyping suggests altered regulation of tau gene expression in progressive supranuclear palsy

被引:128
作者
Rademakers, R
Melquist, S
Cruts, M
Theuns, J
Del-Favero, J
Poorkaj, P
Baker, M
Sleegers, K
Crook, R
De Pooter, T
Kacem, SB
Adamson, J
Van den Bossche, D
Van den Broeck, M
Gass, J
Corsmit, E
De Rijk, P
Thomas, N
Engelborghs, S
Heckman, M
Litvan, I
Crook, J
De Deyn, PP
Dickson, D
Schellenberg, GD
Van Broeckhoven, C
Hutton, ML
机构
[1] Univ Antwerp VIB, Dept Mol Genet, Antwerp, Belgium
[2] Univ Antwerp, Middelheim Hosp, Div Neurol, Antwerp, Belgium
[3] Univ Antwerp, Inst Born Bunge, Lab Neurochem & Behav, Antwerp, Belgium
[4] Mayo Clin, Dept Neurosci, Jacksonville, FL 32224 USA
[5] Mayo Clin, Dept Pathol, Jacksonville, FL 32224 USA
[6] Mayo Clin, Biostat Unit, Jacksonville, FL 32224 USA
[7] Univ Washington, Dept Med, Seattle, WA USA
[8] Univ Washington, Dept Neurol, Seattle, WA 98195 USA
[9] Univ Washington, Dept Pharmacol, Seattle, WA 98195 USA
[10] Univ Washington, Seattle Vet Affairs Med Ctr, Ctr Geriatr Res Educ & Clin, Seattle, WA 98195 USA
[11] Univ Louisville, Sch Med, Louisville, KY 40292 USA
关键词
D O I
10.1093/hmg/ddi361
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two extended haplotypes exist across the tau gene-H1 and H2-with H1 consistently associated with increased risk of progressive supranuclear palsy (PSP). Using 15 haplotype tagging SNPs (htSNPs), capturing > 95% of MAPT haplotype diversity, we performed association analysis in a US sample of 274 predominantly pathologically confirmed PSP patients and 424 matched control individuals. We found that PSP risk is associated with one of two major ancestral H1 haplotypes, H1B, increasing from 14% in control individuals to 22% in PSP patients (P < 0.001). In young PSP patients, the H1B risk could be localized to a 22 kb regulatory region in intron 0 (P < 0.001) and could be fully explained by one SNP, htSNP167, creating a LBP-1c/LSF/CP2 site, shown to regulate the expression of genes in other neurodegenerative disorders. Luciferase reporter data indicated that the 182 bp conserved regulatory region, in which htSNP167 is located, is transcriptionally active with both alleles differentially influencing expression. Further, we replicated the htSNP167 association in a second, independently ascertained US PSP patient-control sample. However, the htSNP association showed that H1 risk alone could not explain the overall differences in H1 and H2 frequencies in PSP patients and control individuals. Thus, risk variants on different H1 htSNP haplotypes and protective variants on H2 contribute to population risk for PSP.
引用
收藏
页码:3281 / 3292
页数:12
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