Amperometric biosensing of carbamate and organophosphate pesticides utilizing screen-printed tyrosinase-modified electrodes

被引:124
作者
Tanimoto de Albuquerque, Yaico D. [1 ]
Franco Ferreira, Lucas [1 ]
机构
[1] Univ Fed Uberlandia, Inst Quim, Lab Electroquim Aplicada, BR-38400089 Uberlandia, MG, Brazil
关键词
amperometric biosensor; tyrosinase electrode; enzyme inhibitors; pesticides;
D O I
10.1016/j.aca.2007.06.013
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A tyrosinase (Tyr) screen-printed biosensor based on the electroreduction of enzymatically generated quinoid products was electrochemically characterized and optimized for determination of carbamates and organophosphorus pesticides. A composite electrode prepared by screen-printing a cobalt (II) phthalocyanine (CoPc) modified cellulose-graphite composite on a polycarbonate support was employed as electrochemical transducer. The Tyr biosensor was prepared by immobilization of enzyme on the composite electrode surface by cross-linking with glutaraldehyde and bovine serum albumin. Parameters affecting the biosensor response such as response time, enzyme loading, concentration and pH of the buffer solution were optimized utilizing catechol as substrate. The maximum response for o-quinone enzymatically generated was obtained after 2 min of reaction. A good reproducibility and high operational stability were found for Tyr biosensor (60 units) at 50 mM phosphate buffer, pH 6.50. Under these conditions, the useful lifetime of biosensor was 10 days. After 15 days, the biosensor could be used with 20% of the initial value. Inhibition studies on the o-quinone steady-state current (at -0.20 V versus Ag/AgCl) were performed to investigate the inhibition kinetics of the pesticides in the enzymatic activity of mushroom tyrosinase. The results shown that the methyl parathion and carbofuran can lead to competitive inhibition process of the enzyme, while diazinon and carbaryl act as mixed inhibitors. Linear relationships were found for methyl parathion (6-100 ppb), diazinon (19-50 ppb), carbofuran (5-90 ppb) and carbaryl (10-50 ppb). Analysis of natural river water samples spiked with 30 ppb of each pesticide showed recoveries between 92.50% and 98.50% and relative standard deviations of 2%. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:210 / 221
页数:12
相关论文
共 52 条
  • [41] Determination of toxic substances based on enzyme inhibition.: Part II.: Electrochemical biosensors for the determination of pesticides using flow systems
    Solé, S
    Merkoçi, A
    Alegret, S
    [J]. CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY, 2003, 33 (02) : 127 - 143
  • [42] Analysis of carbamate pesticides and their metabolites in water by solid phase extraction and liquid chromatography:: A review
    Soriano, JM
    Jiménez, B
    Font, G
    Moltó, JC
    [J]. CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY, 2001, 31 (01) : 19 - 52
  • [43] Application of a sensitive catechol detector for determination of tyrosinase inhibitors
    Streffer, K
    Kaatz, H
    Bauer, CG
    Makower, A
    Schulmeister, T
    Scheller, FW
    Peter, MG
    Wollenberger, U
    [J]. ANALYTICA CHIMICA ACTA, 1998, 362 (01) : 81 - 90
  • [44] PEROXIDASE-INCORPORATED POLYPYRROLE MEMBRANE ELECTRODES
    TATSUMA, T
    GONDAIRA, M
    WATANABE, T
    [J]. ANALYTICAL CHEMISTRY, 1992, 64 (10) : 1183 - 1187
  • [45] THE DEVELOPMENT OF A CATECHOL ENZYME ELECTRODE AND ITS POSSIBLE USE FOR THE DIAGNOSIS AND MONITORING OF NEURAL CREST TUMORS
    TILLYER, CR
    GOBIN, PT
    [J]. BIOSENSORS & BIOELECTRONICS, 1991, 6 (07) : 569 - 573
  • [46] Determination of pesticides using electrochemical biosensors
    Trojanowicz, M
    Hitchman, ML
    [J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1996, 15 (01) : 38 - 45
  • [47] Vanloon GW, 2001, ENV CHEM GLOBAL PERS
  • [48] Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support
    Védrine, C
    Fabiano, S
    Tran-Minh, C
    [J]. TALANTA, 2003, 59 (03) : 535 - 544
  • [49] A comparative study of immobilization methods of a tyrosinase enzyme on electrodes and their application to the detection of dichlorvos organophosphorus insecticide
    Vidal, JC
    Esteban, S
    Gil, J
    Castillo, JR
    [J]. TALANTA, 2006, 68 (03) : 791 - 799
  • [50] VOET D, 2004, BIOCHEMISTRY-US, P473