Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR

被引：340

作者：

Boettcher, Michael ^{[1
]}

McManus, Michael T. ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Microbiol & Immunol, Keck Ctr Noncoding RNA, UCSF Diabet Ctr, San Francisco, CA 94143 USA

来源：

MOLECULAR CELL | 2015年 / 58卷 / 04期

关键词：

ZINC-FINGER NUCLEASES; HUMAN-CELLS; GENOME-WIDE; GENE-EXPRESSION; MAMMALIAN-CELLS; TRANSCRIPTION FACTORS; ENGINEERED NUCLEASES; GUIDED ENDONUCLEASE; CAS NUCLEASES; SPECIFICITY;

D O I：

10.1016/j.molcel.2015.04.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The most widely used approach for defining gene function is to reduce or completely disrupt its normal expression. For over a decade, RNAi has ruled the lab, offering a magic bullet to disrupt gene expression in many organisms. However, new biotechnological tools-specifically CRISPR-based technologies-have become available and are squeezing out RNAi dominance in mammalian cell studies. These seemingly competing technologies leave research investigators with the question: "Which technology should I use in my experiment?" This review offers a practical resource to compare and contrast these technologies, guiding the investigator when and where to use this fantastic array of powerful tools.

引用

页码：575 / 585

页数：11

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