PEGylated lipoplexes: Preparation Protocols affecting DNA condensation and cell Transfection efficiency

被引：17

作者：

Bombelli, Cecilia

Faggioli, Francesca

Luciani, Paola

Mancini, Giovanna

Sacco, Maria Grazia

机构：

[1] Univ Roma La Sapienza, Ist Metodol Chim, CNR, Dipartimento Chim, I-00185 Rome, Italy

[2] Ctr Eccellenza Mat Innovat Nanostrutt Applicaz Ch, I-06123 Perugia, Italy

[3] Commiss European Communities, Joint Res Ctr, Inst Hlth & Consumer Protect, Phys & Chem Exposure Unit, I-21020 Ispra, Italy

来源：

JOURNAL OF MEDICINAL CHEMISTRY | 2007年 / 50卷 / 24期

关键词：

D O I：

10.1021/jm061260f

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a psi phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.

引用

页码：6274 / 6278

页数：5

共 19 条

[1]

ALLEN TM, 1993, LIPOSOME TECHNOLOGY, V3, P59

[2]

Efficient transfection of DNA by liposomes formulated with cationic gemini amphiphiles [J].