Effects of histone acetylation on the solubility and folding of the chromatin fiber

被引：79

作者：

Wang, XY ^{[1
]}

He, C ^{[1
]}

Moore, SC ^{[1
]}

Ausió, J ^{[1
]}

机构：

[1] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC V8W 3P6, Canada

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 16期

关键词：

D O I：

10.1074/jbc.M100501200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The folding ability of chromatin fractions containing approximately identical nucleosome numbers and the same linker histone composition, but with different extents of core histone acetylation, were analyzed by analytical ultracentrifugation. It was found that the acetylated fractions consistently exhibited a relatively small but significantly lower extent of compaction than that of their native nonacetylated counterparts. This was regardless of the extent of the size distribution heterogeneity of the fractions analyzed. Furthermore the acetylated chromatin fibers exhibited an enhanced solubility in both NaCl and MgCl2, which is neither the result of a differential binding affinity of the linker histones to chromatin nor of an alteration in the relative amounts of the histone H1 variants.

引用

页码：12764 / 12768

页数：5

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