Clathrin-dependent and clathrin-independent retrieval of synaptic vesicles in retinal bipolar cells

被引：111

作者：

Jockusch, WJ ^{[1
]}

Praefcke, GJK ^{[1
]}

McMahon, HT ^{[1
]}

Lagnado, L ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

NEURON | 2005年 / 46卷 / 06期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/j.neuron.2005.05.004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Synaptic vesicles can be retrieved rapidly or slowly, but the molecular basis of these kinetic differences has not been defined. We now show that substantially different sets of molecules mediate fast and slow endocytosis in the synaptic terminal of retinal bipolar cells. Capacitance measurements of membrane retrieval were made in terminals in which peptides and protein domains were introduced to disrupt known interactions of clathrin, the AP2 adaptor complex, and amphiphysin. All these manipulations caused a selective inhibition of the slow phase of membrane retrieval (time constant similar to 10 s), leaving the fast phase (similar to 1 s) intact. Slow endocytosis after strong stimulation was therefore dependent on the formation of clathrin-coated membrane. Fast endocytosis occurring after weaker stimuli retrieves vesicle membrane in a clathrin-independent manner. All compensatory endocytosis required GTP hydrolysis, but only a subset of released vesicles were primed for fast, clathrinindependent endocytosis.

引用

页码：869 / 878

页数：10

共 58 条

[31] EpsinR: an AP1/clathrin interacting protein involved in vesicle trafficking [J].