Influenza A virus neuraminidase limits viral superinfection

被引:126
作者
Huang, I-Chueh [1 ]
Li, Wenhui [1 ]
Sui, Jianhua [2 ]
Marasco, Wayne [2 ]
Choe, Hyeryun [3 ]
Farzan, Michael [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Southborough, MA 01772 USA
[2] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Pathol, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Childrens Hosp, Dept Pediat, Boston, MA 02115 USA
关键词
D O I
10.1128/JVI.00079-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Enveloped viruses use multiple mechanisms to inhibit infection of a target cell by more than one virion. These mechanisms may be of particular importance for the evolution of segmented viruses, because superinfection exclusion may limit the frequency of reassortment of viral genes. Here, we show that cellular expression of influenza A virus neuraminidase (NA), but not hemagglutinin (HA) or the M2 proton pump, inhibits entry of HA-pseudotyped retroviruses. Cells infected with H1N1 or H3N2 influenza A virus were similarly refractory to HA-mediated infection and to superinfection with a second influenza A virus. Both HA-mediated entry and viral superinfection were rescued by the neuraminidase inhibitors oseltamivir carboxylate and zanamivir. These inhibitors also prevented the removal of alpha-2,3- and alpha-2,6-linked sialic acid observed in cells expressing NA or infected with influenza A viruses. Our data indicate that NA alone among viral proteins limits influenza A virus superinfection.
引用
收藏
页码:4834 / 4843
页数:10
相关论文
共 56 条
[11]   A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificity [J].
Glaser, L ;
Stevens, J ;
Zamarin, D ;
Wilson, IA ;
García-Sastre, A ;
Tumpey, TM ;
Basler, CF ;
Taubenberger, JK ;
Palese, P .
JOURNAL OF VIROLOGY, 2005, 79 (17) :11533-11536
[12]   Comparison of the activities of zanamivir, oseltamivir, and RWJ-270201 against clinical isolates of influenza virus and neuraminidase inhibitor-resistant variants [J].
Gubareva, LV ;
Webster, RG ;
Hayden, FG .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (12) :3403-3408
[13]   Influenza viruses resistant to the antiviral drug oseltamivir: Transmission studies in ferrets [J].
Herlocher, ML ;
Truscon, R ;
Elias, S ;
Yen, HL ;
Roberts, NA ;
Ohmit, SE ;
Monto, AS .
JOURNAL OF INFECTIOUS DISEASES, 2004, 190 (09) :1627-1630
[14]   PLAQUE-FORMATION WITH INFLUENZA-VIRUSES IN DOG KIDNEY CELLS [J].
HO, PPK ;
YOUNG, AL ;
TRUEHAFT, M .
JOURNAL OF GENERAL VIROLOGY, 1976, 33 (OCT) :143-145
[15]   Mechanism of interference mediated by human parainfluenza virus type 3 infection [J].
Horga, MA ;
Gusella, GL ;
Greengard, O ;
Poltoratskaia, N ;
Porotto, M ;
Moscona, A .
JOURNAL OF VIROLOGY, 2000, 74 (24) :11792-11799
[16]   The H274Y mutation in the influenza A/H1N1 neuraminidase active site following oseltamivir phosphate treatment leave virus severely compromised both in vitro and in vivo [J].
Ives, JAL ;
Carr, JA ;
Mendel, DB ;
Tai, CY ;
Lambkin, R ;
Kelly, L ;
Oxford, JS ;
Hayden, FG ;
Roberts, NA .
ANTIVIRAL RESEARCH, 2002, 55 (02) :307-317
[17]  
KIDO H, 1992, J BIOL CHEM, V267, P13573
[18]  
Kido H, 1999, MOL CELLS, V9, P235
[19]   ANTIVIRAL ACTIVITY OF ANTISERUM SPECIFIC FOR AN INFLUENZA VIRUS NEURAMINIDASE [J].
KILBOURNE, ED ;
LAVER, WG ;
SCHULMAN, JL ;
WEBSTER, RG .
JOURNAL OF VIROLOGY, 1968, 2 (04) :281-+
[20]   Neuraminidase hemadsorption activity, conserved in avian influenza A viruses, does not influence viral replication in ducks [J].
Kobasa, D ;
Rodgers, ME ;
Wells, K ;
Kawaoka, Y .
JOURNAL OF VIROLOGY, 1997, 71 (09) :6706-6713