Influenza A virus neuraminidase limits viral superinfection

被引：126

作者：

Huang, I-Chueh ^{[1
]}

Li, Wenhui ^{[1
]}

Sui, Jianhua ^{[2
]}

Marasco, Wayne ^{[2
]}

Choe, Hyeryun ^{[3
]}

Farzan, Michael ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Southborough, MA 01772 USA

[2] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Pathol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Childrens Hosp, Dept Pediat, Boston, MA 02115 USA

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 10期

关键词：

D O I：

10.1128/JVI.00079-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enveloped viruses use multiple mechanisms to inhibit infection of a target cell by more than one virion. These mechanisms may be of particular importance for the evolution of segmented viruses, because superinfection exclusion may limit the frequency of reassortment of viral genes. Here, we show that cellular expression of influenza A virus neuraminidase (NA), but not hemagglutinin (HA) or the M2 proton pump, inhibits entry of HA-pseudotyped retroviruses. Cells infected with H1N1 or H3N2 influenza A virus were similarly refractory to HA-mediated infection and to superinfection with a second influenza A virus. Both HA-mediated entry and viral superinfection were rescued by the neuraminidase inhibitors oseltamivir carboxylate and zanamivir. These inhibitors also prevented the removal of alpha-2,3- and alpha-2,6-linked sialic acid observed in cells expressing NA or infected with influenza A viruses. Our data indicate that NA alone among viral proteins limits influenza A virus superinfection.

引用

页码：4834 / 4843

页数：10

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