Laser-capture microdissection

被引：477

作者：

Espina, Virginia

Wulfkuhle, Julia D.

Calvert, Valerie S.

VanMeter, Amy

Zhou, Weidong

Coukos, George

Geho, David H.

Petricoin, Emanuel F., III

Liotta, Lance A.

机构：

[1] George Mason Univ, Ctr Appl Proteom & Mol Med, Manassas, VA USA

[2] Univ Penn, Ctr Res Reprod Womens Hlth, Philadelphia, PA 19104 USA

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 02期

关键词：

D O I：

10.1038/nprot.2006.85

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue microecology. Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, RNA transcript profiling, cDNA library generation, proteomics discovery and signal-pathway profiling. Herein we provide a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1 - 1.5 h.

引用

页码：586 / 603

页数：18

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