Determination of the degree of N-acetylation (DA) of chitin and chitosan in the presence of water by first derivative ATR FTIR spectroscopy

被引：60

作者：

Beil, Stephan ^{[1
]}

Schamberger, Anna ^{[1
]}

Naumann, Wolfgang ^{[1
]}

Machill, Susanne ^{[1
]}

van Pee, Karl-Heinz ^{[1
]}

机构：

[1] Tech Univ Dresden, D-01062 Dresden, Germany

来源：

CARBOHYDRATE POLYMERS | 2012年 / 87卷 / 01期

关键词：

Chitosan; Chitin; Degree of N-acetylation; First derivative ATR FTIR spectroscopy; INFRARED-SPECTROSCOPY; DEACETYLATION; SPECTROPHOTOMETRY; SAMPLES;

D O I：

10.1016/j.carbpol.2011.07.025

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A new method for the determination of the degree of N-acetylation (DA) of chitin and chitosan is described using first derivative diamond ATR FTIR spectroscopy. Applying the derivative values of the amide Ill band at 1327 cm(-1) and the CH deformation band of the N-acetyl group at 1383 cm(-1) as measure of the N-acetyl content of the sample in relation to the derivative value of the bridge oxygen vibration at 1163 cm(-1) as internal standard, a linear correlation to the results of first derivative UV spectroscopy was obtained and confirmed by elemental analysis and Raman spectroscopy. The described method allows the determination of the degree of N-acetylation of chitosan and chitin in the presence of water thus making drying procedures unnecessary. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：117 / 122

页数：6

共 16 条

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An infrared investigation in relation with chitin and chitosan characterization [J].