ADP-ribosylation as an intermediate step in inactivation of rifampin by a mycobacterial gene

被引：36

作者：

Quan, S

Imai, T

Mikami, Y

Yazawa, K

Dabbs, ER

Morisaki, N

Iwasaki, S

Hashimoto, Y

Furihata, K

机构：

[1] Chiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Chuo Ku, Chiba 2608673, Japan

[2] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 113, Japan

[3] Univ Tokyo, Dept Agr Chem, Bunkyo Ku, Tokyo 113, Japan

[4] Univ Witwatersrand, Dept Genet, ZA-2050 Johannesburg, South Africa

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 01期

关键词：

D O I：

10.1128/AAC.43.1.181

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacterium smegmatis DSM43756 inactivates rifampin, and the inactivated antibiotic product recovered from culture medium was ribosylated on the 23-OH group. To study this process, the gene responsible for the inactivation was expressed at high levels by the lac promoter in Escherichia coli conferring resistance to >500 mu g of antibiotic per mi. Cell homogenates generated a novel derivative designated RIP-TAs; in this study, we determined that RIP-TAs is 23 (O-ADP-ribosyl)rifampin. Our results indicated that RIP-TAs is an intermediate in the pathway leading to ribosylated rifampin and that the previously characterized gene encodes a mono(ADP-ribosyl)transferase which, however, shows no sequence similarity to other enzymes of this class.

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页码：181 / 184

页数：4

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