Transmission of human TT virus of genotype 1a to chimpanzees with fecal supernatant or serum from patients with acute TTV infection

被引：42

作者：

Tawara, A

Akahane, Y

Takahashi, M

Nishizawa, T

Ishikawa, T

Okamoto, H ^{[1
]}

机构：

[1] Jichi Med Sch, Div Immunol, Minami Kawachi, Tochigi 3290498, Japan

[2] Jichi Med Sch, Div Mol Virol, Minami Kawachi, Tochigi 3290498, Japan

[3] Jichi Med Sch, Dept Pediat, Minami Kawachi, Tochigi 3290498, Japan

[4] Yamanashi Med Univ, Dept Internal Med 1, Yamanashi 4093898, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 278卷 / 02期

关键词：

TT virus; chimpanzee; experimental infection; feces; alanine aminotransferase; genotypes; hepatitis viruses;

D O I：

10.1006/bbrc.2000.3825

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fecal supernatant or serum containing TT virus (TTV) of genotype la (10(5) copies/ml) from patients with acute TTV infection was inoculated intravenously into two naive chimpanzees. Serum samples were obtained weekly and tested for TTV DNA by genotype 1-specific polymerase chain reaction. TTV DNA was detected in chimpanzee 228 at weeks 5-15 after inoculation with 0.5 mi of serum, and in chimpanzee 234 at weeks 7-19 after inoculation with 1 mi of fecal supernatant. The TTV DNA titer peaked at weeks 12 and 13 in chimpanzee 228 and at weeks 14-16 in chimpanzee 234. Mild biochemical and histological changes in biopsied liver samples were observed in both chimpanzees in association with the reduction in TTV titer. TTV DNA was transient in chimpanzee 228, but in chimpanzee 234 it reappeared at week 21 and persisted through week 30. These results indicate that TTV in feces is infectious and suggest that ITV has hepatitis-inducing capacity. (C) 2000 Academic Press.

引用

页码：470 / 476

页数：7

共 47 条

[1] TT virus infection in nonhuman primates and characterization of the viral genome: Identification of simian TT virus isolates [J].