Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124

被引：32

作者：

Dekker, B

Keen, H

Shaw, D

Disley, L

Hastings, D

Hadfield, J

Reader, A

Allan, D

Julyan, P

Watson, A

Zweit, J ^{[1
]}

机构：

[1] Christie Hosp NHS Trust, Paterson Inst Canc Res, CRUK UMIST Dept Radiochem Targeting & Imaging, Manchester M20 4BX, Lancs, England

[2] Univ Manchester, Inst Sci & Technol, Dept Instrumentat & Analyt Sci, Manchester M60 1QD, Lancs, England

[3] Paterson Inst Canc Res, CRUK Dept Immunol, Manchester M20 4BX, Lancs, England

[4] Christie Hosp NHS Trust, NW Med Phys, Manchester M20 4BX, Lancs, England

[5] Univ Salford, Sch Environm & Life Sci, Salford M5 4WT, Lancs, England

[6] Paterson Inst Canc Res, Phys & Elect Unit, Manchester M20 4BX, Lancs, England

[7] Univ Liverpool, Dept Med, Liverpool L69 3GA, Merseyside, England

来源：

NUCLEAR MEDICINE AND BIOLOGY | 2005年 / 32卷 / 04期

关键词：

apoptosis; annexin V; molecular imaging; positron emission tomography; iodine-124;

D O I：

10.1016/j.nucmedbio.2005.02.002

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

We are interested in imaging cell death in vivo using annexin V radiolabeled with I-124. In this study, [I-124]4IB-annexin V and [I-124]4IB-ovalbumin were made using [I-124]N-hydroxysuccinimdyl-4-iodobenzoate prepared by iododestannylation of N-hydroxysuccimmidyl-4-(ttributystannyl)benzoate. [I-124]4IB-annexin V binds to phosphatidylserine-coated microliter plates and apoptotic Jurkat cells and accumulates in hepatic apoptotic lesions in mice treated with anti-Fas antibody, while [I-124]4IB-ovalbumin does not. In comparison with I-124-annexin V,[I-124]4IB-annexin V has a higher rate of binding to phospliatidylserine in vitro, a higher kidney and urine uptake, a lower thyroid and stomach content uptake, greater plasma stability and a lower rate of plasma clearance. Binding of radioactivity to apoptotic cells relative to normal cells in vitro and in vivo appears to be lower for[I-124]4IB-annexin V than for I-124-annexin V. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：403 / 413

页数：11