A DREB gene from Limonium bicolor mediates molecular and physiological responses to copper stress in transgenic tobacco

被引：52

作者：

Ban, Qiaoying ^{[1
]}

Liu, Guifeng ^{[1
]}

Wang, Yucheng ^{[1
]}

机构：

[1] NE Forestry Univ, Minist Educ, Key Lab Forest Tree Genet Improvement & Biotechno, Harbin 150040, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2011年 / 168卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Copper stress; DREB; Gene expression regulation; Limonium bicolor; Stress tolerance; BINDING TRANSCRIPTION FACTOR; TRANSLATION INITIATION-FACTOR; ORYZA-SATIVA L; SALT STRESS; ENHANCED RESISTANCE; LEA PROTEINS; TOLERANCE; RICE; DROUGHT; EXPRESSION;

D O I：

10.1016/j.jplph.2010.08.013

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The DRE-binding (DREB) transcription factors play an important role in regulating stress-related genes. In the present study, a novel DREB gene (LbDREB) from Limonium bicolor was cloned. To characterize the function of DREB in heavy metal stress tolerance, LbDREB-transformed tobacco plants were generated and subjected to CuSO4 stress. Analysis of the role of LbDREB in tolerance to copper stress in transgenic tobacco showed that overexpression of LbDREB increased the contents of soluble protein and proline, and elevated the ratio of K to Na under CuSO4 stress. Moreover, overexpression of LbDREB can up-regulate some stress-related genes, including Cu/Zn superoxide dismutase (Cu/Zn SOD), peroxidases (PODs), late embryogenesis abundant (LEA), and lipid transfer proteins (LTP). These results suggest that LbDREB can enhance plant copper tolerance by up-regulating a series of stress-related genes, thereby mediating physiological processes associated with stress tolerance in plants. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：449 / 458

页数：10

共 46 条

[1] Overexpression of PgDREB2A transcription factor enhances abiotic stress tolerance and activates downstream stress-responsive genes [J].

Agarwal, Parinita ;

Agarwal, Pradeep K. ;

Joshi, Arvind J. ;

Sopory, Sudhir K. ;

Reddy, Malireddy K. .

MOLECULAR BIOLOGY REPORTS, 2010, 37 (02) :1125-1135

[2] RAPID DETERMINATION OF FREE PROLINE FOR WATER-STRESS STUDIES [J].

BATES, LS ;

WALDREN, RP ;

TEARE, ID .

PLANT AND SOIL, 1973, 39 (01) :205-207

[3] Improved analytical methods for determination of nitrogenous stress metabolites occurring in Limonium species [J].

Bouchereau, A ;

Duhazé, C ;

Martin-Tanguy, J ;

Guégan, JP ;

Larher, F .

JOURNAL OF CHROMATOGRAPHY A, 1999, 836 (02) :209-221

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean (Glycine max L.) [J].

Chen, Ming ;

Xu, Zhaoshi ;

Xia, Lanqin ;

Li, Liancheng ;

Cheng, Xianguo ;

Dong, Jianhui ;

Wang, Qiaoyan ;

Ma, Youzhi .

JOURNAL OF EXPERIMENTAL BOTANY, 2009, 60 (01) :121-135

[6] GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants [J].

Chen, Ming ;

Wang, Qiao-Yan ;

Cheng, Xian-Guo ;

Xu, Zhao-Shi ;

Li, an-Cheng Li ;

Ye, Xing-Guo ;

Xia, Lan-Qin ;

Ma, You-Zhi .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 353 (02) :299-305

[7] Isolation and characterization of multiple abundant lipid transfer protein isoforms in developing sesame (Sesamum indicum L.) seeds [J].

Choi, Ah Mi ;

Lee, Saet Buyl ;

Cho, Sung Ho ;

Hwang, Inhwan ;

Hur, Cheol-Goo ;

Suh, Mi Chung .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2008, 46 (02) :127-139

[8] Accumulation of LEA proteins in salt (NaCl) stressed young seedlings of rice (Oryza sativa L.) cultivar Bura Rata and their degradation during recovery from salinity stress [J].

Chourey, K ;

Ramani, S ;

Apte, SK .

JOURNAL OF PLANT PHYSIOLOGY, 2003, 160 (10) :1165-1174

[9] Characterization of the novel gene BjDREB1B encoding a DRE-binding transcription factor from Brassica juncea L. [J].

Cong, Lin ;

Chai, Tuan-Yao ;

Zhang, Yu-Xiu .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 371 (04) :702-706

[10] Regulatory and structural properties differentiating the chromosomal and the bacteriophage-associated Escherichia coli O157:H7 Cu, Zn Superoxide Dismutases [J].

D'Orazio, Melania ;

Scotti, Raffaella ;

Nicolini, Laura ;

Cervoni, Laura ;

Rotilio, Giuseppe ;

Battistoni, Andrea ;

Gabbianelli, Roberta .

BMC MICROBIOLOGY, 2008, 8 (1)

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