A DREB gene from Limonium bicolor mediates molecular and physiological responses to copper stress in transgenic tobacco

被引：52

作者：

Ban, Qiaoying ^{[1
]}

Liu, Guifeng ^{[1
]}

Wang, Yucheng ^{[1
]}

机构：

[1] NE Forestry Univ, Minist Educ, Key Lab Forest Tree Genet Improvement & Biotechno, Harbin 150040, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2011年 / 168卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Copper stress; DREB; Gene expression regulation; Limonium bicolor; Stress tolerance; BINDING TRANSCRIPTION FACTOR; TRANSLATION INITIATION-FACTOR; ORYZA-SATIVA L; SALT STRESS; ENHANCED RESISTANCE; LEA PROTEINS; TOLERANCE; RICE; DROUGHT; EXPRESSION;

D O I：

10.1016/j.jplph.2010.08.013

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The DRE-binding (DREB) transcription factors play an important role in regulating stress-related genes. In the present study, a novel DREB gene (LbDREB) from Limonium bicolor was cloned. To characterize the function of DREB in heavy metal stress tolerance, LbDREB-transformed tobacco plants were generated and subjected to CuSO4 stress. Analysis of the role of LbDREB in tolerance to copper stress in transgenic tobacco showed that overexpression of LbDREB increased the contents of soluble protein and proline, and elevated the ratio of K to Na under CuSO4 stress. Moreover, overexpression of LbDREB can up-regulate some stress-related genes, including Cu/Zn superoxide dismutase (Cu/Zn SOD), peroxidases (PODs), late embryogenesis abundant (LEA), and lipid transfer proteins (LTP). These results suggest that LbDREB can enhance plant copper tolerance by up-regulating a series of stress-related genes, thereby mediating physiological processes associated with stress tolerance in plants. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：449 / 458

页数：10

共 46 条

[11] OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression [J].