A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection

被引:172
作者
Zhou, Yuntao [1 ]
Huang, Qing [1 ]
Gao, Jimin [2 ]
Lu, Jianxin [2 ]
Shen, Xizhong [3 ]
Fan, Chunhai [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China
[2] Wenzhou Med Coll, Wenzhou 325035, Zhejiang, Peoples R China
[3] Fudan Univ, Zhongshan Hosp, Shanghai 200032, Peoples R China
关键词
EXPRESSION; DNA; SEQUENCE; ARRAY; PCR;
D O I
10.1093/nar/gkq556
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples.
引用
收藏
页码:e156 / e156
页数:5
相关论文
共 37 条
[1]   microRNAs: Tiny regulators with great potential [J].
Ambros, V .
CELL, 2001, 107 (07) :823-826
[2]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[3]   Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis [J].
Berezikov, Eugene ;
van Tetering, Geert ;
Verheul, Mark ;
van de Belt, Jose ;
van Laake, Linda ;
Vos, Joost ;
Verloop, Robert ;
van de Wetering, Marc ;
Guryev, Victor ;
Takada, Shuji ;
van Zonneveld, Anton Jan ;
Mano, Hiroyuki ;
Plasterk, Ronald ;
Cuppen, Edwin .
GENOME RESEARCH, 2006, 16 (10) :1289-1298
[4]   MicroRNA expression profiling of human breast cancer identifies new markers of tumor subtype [J].
Blenkiron, Cherie ;
Goldstein, Leonard D. ;
Thorne, Natalie P. ;
Spiteri, Inmaculada ;
Chin, Suet-Feung ;
Dunning, Mark J. ;
Barbosa-Morais, Nuno L. ;
Teschendorff, Andrew E. ;
Green, Andrew R. ;
Ellis, Ian O. ;
Tavare, Simon ;
Caldas, Carlos ;
Miska, Eric A. .
GENOME BIOLOGY, 2007, 8 (10)
[5]   Thermodynamic basis of the enhanced specificity of structured DNA probes [J].
Bonnet, G ;
Tyagi, S ;
Libchaber, A ;
Kramer, FR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6171-6176
[6]   MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias [J].
Calin, GA ;
Liu, CG ;
Sevignani, C ;
Ferracin, M ;
Felli, N ;
Dumitru, CD ;
Shimizu, M ;
Cimmino, A ;
Zupo, S ;
Dono, M ;
Dell'Aquila, ML ;
Alder, H ;
Rassenti, L ;
Kipps, TJ ;
Bullrich, F ;
Negrini, M ;
Croce, CM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (32) :11755-11760
[7]   A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA) [J].
Castoldi, M ;
Schmidt, S ;
Benes, V ;
Noerholm, M ;
Kulozik, AE ;
Hentze, MW ;
Muckenthaler, MU .
RNA, 2006, 12 (05) :913-920
[8]   Real-time quantification of microRNAs by stem-loop RT-PCR [J].
Chen, CF ;
Ridzon, DA ;
Broomer, AJ ;
Zhou, ZH ;
Lee, DH ;
Nguyen, JT ;
Barbisin, M ;
Xu, NL ;
Mahuvakar, VR ;
Andersen, MR ;
Lao, KQ ;
Livak, KJ ;
Guegler, KJ .
NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9
[9]   Highly sensitive and specific microRNA expression profiling using BeadArray technology [J].
Chen, Jing ;
Lozach, Jean ;
Wickham, Eliza ;
Barnes, Garcia Bret ;
Luo, Shujun ;
Mikoulitch, Ivan ;
Zhou, Lixin ;
Schroth, Gary ;
Fan, Jian-Bing .
NUCLEIC ACIDS RESEARCH, 2008, 36 (14)
[10]   Highly Sensitive Determination of microRNA Using Target-Primed and Branched Rolling-Circle Amplification [J].
Cheng, Yongqiang ;
Zhang, Xian ;
Li, Zhengping ;
Jiao, Xiaoxia ;
Wang, Yucong ;
Zhang, Yali .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (18) :3268-3272