Microbial transformation of methyl protodioscin by Cunninghamella elegans

被引：16

作者：

He, XJ ^{[1
]}

Wang, XL

Liu, B

Su, LN

Wang, GH

Qu, GX

Yao, ZH

Liu, RH

Yao, XS

机构：

[1] Jinan Univ, Inst Trad Chinese Med & Nat Proc, Guangzhou 510632, Peoples R China

[2] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA

[3] Shenyang Pharmaceut Univ, Dept Nat Prod Chem, Shenyang 110016, Peoples R China

[4] Univ Exeter, Dept Bioinformat, Exeter EX4 6TJ, Devon, England

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2005年 / 35卷 / 1-3期

基金：

中国国家自然科学基金;

关键词：

methyl protodioscin; microbial transformation; Cunninghamella elegans; anticancer compound; cytotoxicity;

D O I：

10.1016/j.molcatb.2005.05.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Biotransformation of methyl protodioscin (1) by Cunninghamella elegans (AS 3.1207) was investigated. Nine bioconversion products were isolated and identified. Eight of the bioconversion products were pregnane glycoside or steroidal saponins. It was found that steroidal saponin skeleton could be converted to pregnenolone skeleton only using microbial methods, which must have chemical procedures in the reported literatures. The found enriched the types of bioconversion reaction and provided a new way for the production of androstenedione. Most bioconversion products showed considerable cytotoxic activities against HepG2, NCI-H460, MCF-7 and HeLa cell lines compared to methyl protodioscin. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：33 / 40

页数：8

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