Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells

被引：909

作者：

Huang, Bo ^{[1
,2
]}

Babcock, Hazen

Zhuang, Xiaowei ^{[3
,4
,5
]}

机构：

[1] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA

[3] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA

[4] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[5] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

CELL | 2010年 / 143卷 / 07期

基金：

美国国家卫生研究院;

关键词：

STED MICROSCOPY REVEALS; FLUORESCENCE MICROSCOPY; STIMULATED-EMISSION; 3-DIMENSIONAL SUPERRESOLUTION; SUBDIFFRACTION RESOLUTION; EXCITATION MICROSCOPY; LIVING CELLS; LOCALIZATION; NANOSCOPY; LIMIT;

D O I：

10.1016/j.cell.2010.12.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anyone who has used a light microscope has wished that its resolution could be a little better. Now, after centuries of gradual improvements, fluorescence microscopy has made a quantum leap in its resolving power due, in large part, to advancements over the past several years in a new area of research called super-resolution fluorescence microscopy. In this Primer, we explain the principles of various super-resolution approaches, such as STED, (S) SIM, and STORM/(F) PALM. Then, we describe recent applications of super-resolution microscopy in cells, which demonstrate how these approaches are beginning to provide new insights into cell biology, microbiology, and neurobiology.

引用

页码：1047 / 1058

页数：12

共 90 条

[1]

Abbe E., 1873, Archiv f ur Mikroskopische Anatomie, V9, P413, DOI DOI 10.1007/BF02956173

[2] Multicolor super-resolution imaging with photo-switchable fluorescent probes [J].