Synthesis, encapsulation, purification and coupling of single quantum dots in phospholipid micelles for their use in cellular and in vivo imaging

被引：142

作者：

Carion, Olivier ^{[1
]}

Mahler, Benoit ^{[1
]}

Pons, Thomas ^{[1
]}

Dubertret, Benoit ^{[1
]}

机构：

[1] Ecole Super Phys & Chim Ind Ville Paris, CNRS, UPRA5, Lab Photon & Mat, F-75231 Paris 05, France

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 10期

关键词：

D O I：

10.1038/nprot.2007.351

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A detailed protocol for the synthesis of core/shell semiconductor nanocrystal, their encapsulation into phospholipid micelles, their purification and their coupling to a controlled number of small molecules is given. The protocol for the core/shell quantum dot (QD) CdSe/CdZnS synthesis has been specifically designed with two constraints in mind: green and reproducible core/shell QD synthesis with thick shell structure and QDs that can easily be encapsulated in poly(ethylene glycol)-phospholipid micelles with one QD per micelle. We present two procedures for the QD purification that are suitable for the use of QD micelles for in vivo imaging: ultracentrifugation and size-exclusion chromatography. We also discuss the different coupling chemistry for covalently linking a controlled number of molecules to the QD micelles. The total time durations for the different protocols are as follows: QD synthesis: 6 h; encapsulation: 15 min; purification: 1-4 h; coupling: reaction dependent.

引用

页码：2383 / 2390

页数：8

共 29 条

[11] Spherical micelles and other self-assembled structures in dilute aqueous mixtures of poly(ethylene glycol) lipids [J].

Johnsson, M ;

Hansson, P ;

Edwards, K .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (35) :8420-8430

[12] Synthesis of multi-shell nanocrystals by a single step coating process [J].

Jun, Shinae ;

Jang, Eunjoo ;

Lim, Jung Eun .

NANOTECHNOLOGY, 2006, 17 (15) :3892-3896

[13] On the absorption cross section of CdSe nanocrystal quantum dots [J].

Leatherdale, CA ;

Woo, WK ;

Mikulec, FV ;

Bawendi, MG .

JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (31) :7619-7622

[14] Large-scale synthesis of nearly monodisperse CdSe/CdS core/shell nanocrystals using air-stable reagents via successive ion layer adsorption and reaction [J].

Li, JJ ;

Wang, YA ;

Guo, WZ ;

Keay, JC ;

Mishima, TD ;

Johnson, MB ;

Peng, XG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (41) :12567-12575

[15] Mechanistic study of precursor evolution in colloidal group II-VI semiconductor nanocrystal synthesis [J].

Liu, Haitao ;

Owen, Jonathan S. ;

Alivisatos, A. Paul .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (02) :305-312

[16] Quantum dot bioconjugates for imaging, labelling and sensing [J].

Medintz, IL ;

Uyeda, HT ;

Goldman, ER ;

Mattoussi, H .

NATURE MATERIALS, 2005, 4 (06) :435-446

[17] Quantum dots for live cells, in vivo imaging, and diagnostics [J].

Michalet, X ;

Pinaud, FF ;

Bentolila, LA ;

Tsay, JM ;

Doose, S ;

Li, JJ ;

Sundaresan, G ;

Wu, AM ;

Gambhir, SS ;

Weiss, S .

SCIENCE, 2005, 307 (5709) :538-544

[18] SYNTHESIS AND CHARACTERIZATION OF NEARLY MONODISPERSE CDE (E = S, SE, TE) SEMICONDUCTOR NANOCRYSTALLITES [J].

MURRAY, CB ;

NORRIS, DJ ;

BAWENDI, MG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (19) :8706-8715

[19] Synthesis and characterization of monodisperse nanocrystals and close-packed nanocrystal assemblies [J].

Murray, CB ;

Kagan, CR ;

Bawendi, MG .

ANNUAL REVIEW OF MATERIALS SCIENCE, 2000, 30 :545-610

[20] Hydrodynamic dimensions, electrophoretic mobility, and stability of hydrophilic quantum dots [J].

Pons, Thomas ;

Uyeda, H. Tetsuo ;

Medintz, Igor L. ;

Mattoussi, Hedi .

JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (41) :20308-20316

← 1 2 3 →