A micromachined capillary electrophoresis chip with fully integrated electrodes for separation and electrochemical detection

被引:38
作者
Wilke, R [1 ]
Büttgenbach, S [1 ]
机构
[1] Tech Univ Braunschweig, Inst Microtechnol, D-38124 Braunschweig, Germany
关键词
lab-on-a-chip; micro total analysis system (mu TAS); capillary electrophoresis; electrochemical detection;
D O I
10.1016/S0956-5663(03)00178-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A novel analytical microsystem with fully integrated electrodes for electrophoresis and amperometrical detection is described. With respect to the lab-on-a-chip concept a capillary electrophoresis (CE) microsystem has been fabricated with a total of six gold electrodes for sample injection, separation and electrochemical detection using standard microfabrication technologies. The device is a ready-to-use system that does not need any extra mechanical apparatus for electrode insertion. The CE-chip has successfully been tested by measuring hydrogen peroxide, ascorbic acid and uric acid simultaneously. All three oxidizable species could be detected in less than 70 s. Glucose was detected by performing an enzymatic reaction along the separation channel. The microsystem showed a very good reproducibility. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:149 / 153
页数:5
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