A Smart DNAzyme-MnO2 Nanosystem for Efficient Gene Silencing

被引：301

作者：

Fan, Huanhuan ^{[1
]}

Zhao, Zilong ^{[1
]}

Yan, Guobei ^{[1
]}

Zhang, Xiaobing ^{[1
]}

Yang, Chao ^{[1
]}

Meng, Hongmin ^{[1
]}

Chen, Zhuo ^{[1
]}

Liu, Hui ^{[1
]}

Tan, Weihong ^{[1
]}

机构：

[1] Hunan Univ, Innovat Ctr Chem & Mol Med,Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn,Coll Biol Collaborat, Changsha 410082, Hunan, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 16期

关键词：

DNAzyme; gene silencing; manganese; nanotechnology; photodynamic therapy; MNO2; NANOSHEET; LEAD; SENSOR; PROBE; SITE; ION;

D O I：

10.1002/anie.201411417

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

DNAzymes hold promise for gene-silencing therapy, but the lack of sufficient cofactors in the cell cytoplasm, poor membrane permeability, and poor biostability have limited the use of DNAzymes in therapeutics. We report a DNAzyme-MnO2 nanosystem for gene-silencing therapy. MnO2 nanosheets adsorb chlorin e6-labelled DNAzymes (Ce6), protect them from enzymatic digestion, and efficiently deliver them into cells. The nanosystem can also inhibit O-1(2) generation by Ce6 in the circulatory system. In the presence of intracellular glutathione (GSH), MnO2 is reduced to Mn(2+)ions, which serve as cofactors of 10-23DNAzyme for gene silencing. The release of Ce6 generates O-1(2) for more efficient photodynamic therapy. The Mn(2+)ions also enhance magnetic resonance contrast, providing GSH-activated magnetic resonance imaging (MRI) of tumor cells. The integration of fluorescence recovery and MRI activation provides fluorescence/MRI bimodality for monitoring the delivery of DNAzymes.

引用

页码：4801 / 4805

页数：5

共 35 条

[1]

Hairpin DNAzymes: a new toot for efficient cellular gene silencing [J].