Hop/STI1 modulates retinal proliferation and cell death independent of PrPC

被引：18

作者：

Arruda-Carvalho, Maithe ^{[1
]}

Njaine, Brian ^{[1
]}

Silveira, Mariana S. ^{[1
]}

Linden, Rafael ^{[1
]}

Chiarini, Luciana B. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biofis, Ctr Ciencias Saude, BR-21941902 Rio De Janeiro, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 361卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

co-chaperone; development; retina; proliferation; programmed cell death; prion protein;

D O I：

10.1016/j.bbrc.2007.07.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hop/STI1 is a co-chaperone adaptor protein for Hsp70/Hsp90 complexes. Hop/STI1 is found extracellularly and modulates cell death and differentiation through interaction with the prion protein (PrPc). Here, we investigated the expression of hop/STI1 and its role upon cell proliferation and cell death in the developing retina. Hop/STI1 is more expressed in developing rat retina than in the mature tissue. Hop/STI1 blocks retinal cell death in the neuroblastic layer (NBL) in a PrPC dependent manner, but failed to protect ganglion cells against axotomy-induced cell death. An antibody raised against hop/STI1 (alpha-STI1) blocked both ganglion cell and NBL cell death independent of PrPc. cAMP/PKA, ERK, PI3K and PKC signaling pathways were not involved in these effects. Hop/STI1 treatment reduced proliferation, while alpha-STI1 increased proliferation in the developing retina, both independent of PrPc. We conclude that hop/STI1 can modulate both proliferation and cell death in the developing retina independent of PrPc. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：474 / 480

页数：7

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