Regulation of a blood-brain barrier-specific enzyme expressed by cerebral pericytes (pericytic aminopeptidase N pAPN) under cell culture conditions

被引:31
作者
Ramsauer, M [1 ]
Kunz, J [1 ]
Krause, D [1 ]
Dermietzel, R [1 ]
机构
[1] Ruhr Univ Bochum, Inst Anat, D-4630 Bochum, Germany
关键词
blood-brain barrier; pericytes; aminopeptidase N; in vitro regulation; coculture conditions;
D O I
10.1097/00004647-199811000-00014
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In this study we show that the aminopeptidase N of cerebral pericytes (pAPN) associated with the blood-brain barrier (BBB) is downregulated in pericytic cell cultures. This observation is in accordance with previous data describing comparable in vitro, effects for BBB-specific enzymes of endothelial or pericytic origin, such as gamma-glutamyl transpeptidase or alkaline phosphatase. By polymerase chain reaction and in situ hybridization we were able to determine that the downregulation of pAPN occurs at the posttranscriptional level. The mRNA of pAPN was found to be constitutively expressed even when the protein is no longer detectable. Culturing the pericytes in an endothelial cell-conditioned medium allowed pAPN to be reexpressed. However, the reexpression effect depended largely on the culturing conditions of the pericytes. Although purified pericytes deprived of endothelial cells did not reveal a reexpression effect, pericytes that were kept in contact with endothelial cells were able to acquire a pAPN-positive phenotype, indicating that endothelial cells constitute an essential requirement for the in vitro reexpression of pAPN. Astrocytes, however, were insufficient in exerting any reexpression effect.
引用
收藏
页码:1270 / 1281
页数:12
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