Characterization of a heavy metal ion transporter in the lysosomal membrane

被引：38

作者：

Havelaar, AC ^{[1
]}

de Gast, IL ^{[1
]}

Snijders, S ^{[1
]}

Beerens, CEMT ^{[1
]}

Mancini, GMS ^{[1
]}

Verheijen, FW ^{[1
]}

机构：

[1] Erasmus Univ, Dept Clin Genet, NL-3015 GE Rotterdam, Netherlands

来源：

FEBS LETTERS | 1998年 / 436卷 / 02期

关键词：

lysosomal transporter; heavy metal ion; copper; silver; P-type ATPase; Wilson disease;

D O I：

10.1016/S0014-5793(98)01133-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lysosomes are thought to play a role in various aspects of heavy metal metabolism. In the present study we demonstrate for the first time the presence of a heavy metal ion transport protein in the lysosomal membrane. Uptake of radioactive silver both in highly purified lysosomal membrane vesicles and in purified intact lysosomes showed the typical kinetics of a carrier-mediated process. This transport was stimulated by ATP hydrolysis, and showed specificity for Ag+ Cu2+, and Cd2+. All biochemical properties of this lysosomal metal ion transporter could classify it as a heavy metal transporting P-type ATPase. Long Evans Cinnamon (LEC) rats, an animal model for the copper transport disorder Wilson disease, shelved normal lysosomal silver transport. (C) 1998 Federation of European Biochemical Societies.

引用

页码：223 / 227

页数：5

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